NGS Details (SRR3458562_Metagenome_example): Escherichia virus Lambda_4A7

Assembly

17853 (69 contig(s))
7.3
1258
81.17 rpm (after QC)
0
read mapping against reference + variant calling
Bcf Tools

Coverage Map

Variant Tables

Assignment

Escherichia virus Lambda_4A7 (Taxonomy ID: 2686055)
99.9104
99.153
24
70

Alignment

35642.0 (NT) + 38208.0 (AA) = 73850.0
99.8769
Local, heuristic, nucleotide (BLASTN)

Alignment Detailed Statistics

BeginEndCoverageScoreConcordanceMatchesIdentitiesI/D/M/F*Stop Codons
NT24916836.3%3564299.8%17853 (100%)17837 (99.9%)0/0
Mutations:624C>T, 635G>T, 1796T>C, 2051C>T, 2075T>G, 2249T>C, 5628C>T, 5647G>T, 5661T>A, 13270C>A, 13297A>G, 13345A>G, 28568C>T, 28643T>C, 48851G>T, 48872G>A
CDS
1150100%965100%150 (100%)150 (100%)0/0/0/01
1643100%456399.8%643 (100%)642 (99.8%)0/0/0/01
Protein mutations:D551E (2075T>G)
Codon mutations:CTG68TTG (624C>T), ACG71ACT (635G>T), ACT458ACC (1796T>C), GCC543GCT (2051C>T), GAT551GAG (2075T>G), GAT609GAC (2249T>C)
16695.7%40698.8%66 (100%)65 (98.5%)0/0/0/00
953196.0%352398.6%510 (100%)508 (99.6%)0/0/0/01
150266.3%212798.2%333 (100%)330 (99.1%)0/0/0/01
1116100%737100%116 (100%)116 (100%)0/0/0/01
2634388.3%208399.7%303 (100%)303 (100%)0/0/0/01
1141100%928100%141 (100%)141 (100%)0/0/0/01
111294.9%806100%112 (100%)112 (100%)0/0/0/00
11317130.6%40696.7%59 (100%)58 (98.3%)0/0/0/00
10613220.5%19197.9%27 (100%)27 (100%)0/0/0/01
18722114.2%19591.5%35 (100%)34 (97.1%)0/0/0/00
9511112.1%10090.9%17 (100%)16 (94.1%)0/0/0/00
244213.1%13496.4%19 (100%)18 (94.7%)0/0/0/00
3485438.1%43094.1%69 (100%)67 (97.1%)0/0/0/00
2211055.5%41892.3%61 (100%)58 (95.1%)0/0/0/02
Protein mutations:S90* (13270C>A), E99G (13297A>G)
Codon mutations:TCG90TAG (13270C>A), GAG99GGG (13297A>G)
114251.1%83096.8%119 (100%)116 (97.5%)0/0/0/00
14322323.0%45192.4%57 (100%)56 (98.2%)0/0/0/00
9511712.0%12788.2%23 (100%)22 (95.7%)0/0/0/00
60103127.4%196396.5%311 (100%)306 (98.4%)0/0/0/00
156046.0%186797.1%296 (100%)292 (98.6%)0/0/0/00
47753811.5%45797.9%62 (100%)62 (100%)0/0/0/01
14388.4%23195.9%38 (100%)37 (97.4%)0/0/0/01
789317.2%10791.5%16 (100%)16 (100%)0/0/0/01
19864.3%50794.1%63 (100%)61 (96.8%)0/0/0/01
128686.0%158999.5%246 (100%)246 (100%)0/0/0/01
128686.0%163198.0%246 (100%)245 (99.6%)0/0/0/01
2927681.5%144697.8%225 (100%)224 (99.6%)0/0/0/01
3423461.3%129098.0%187 (100%)185 (98.9%)0/0/0/00
151100%325100%51 (100%)51 (100%)0/0/0/01
138176.1%203498.3%290 (100%)287 (99.0%)0/0/0/01
67988.6%49497.2%70 (100%)70 (100%)0/0/0/01
35496.6%9596.0%15 (100%)15 (100%)0/0/0/00
14517110.3%20296.2%27 (100%)27 (100%)0/0/0/00
2012043.2%71199.4%101 (100%)100 (99.0%)0/0/0/00
110594.6%73199.5%105 (100%)104 (99.0%)0/0/0/00
10412819.5%153100%25 (100%)25 (100%)0/0/0/01
736179.5%192698.4%287 (100%)284 (99.0%)0/0/0/01
15662.5%28094.9%45 (100%)43 (95.6%)0/0/0/00
1621663.0%4591.8%5 (100%)5 (100%)0/0/0/01
13120.9%21597.3%31 (100%)30 (96.8%)0/0/0/00
165100%489100%65 (100%)65 (100%)0/0/0/01
Proteins
1150100%965100%150 (100%)150 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
1643100%456399.8%643 (100%)642 (99.8%)0/0/0/01
Protein mutations:D551E (2075T>G)
Codon mutations:CTG68TTG (624C>T), ACG71ACT (635G>T), ACT458ACC (1796T>C), GCC543GCT (2051C>T), GAT551GAG (2075T>G), GAT609GAC (2249T>C)
16695.7%40698.8%66 (100%)65 (98.5%)0/0/0/00
Protein mutations:none
Codon mutations:none
953196.0%352398.6%510 (100%)508 (99.6%)0/0/0/01
Protein mutations:none
Codon mutations:none
150266.3%212798.2%333 (100%)330 (99.1%)0/0/0/01
Protein mutations:none
Codon mutations:none
1116100%737100%116 (100%)116 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
2634388.3%208399.7%303 (100%)303 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
1141100%928100%141 (100%)141 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
111294.9%806100%112 (100%)112 (100%)0/0/0/00
Protein mutations:none
Codon mutations:none
11317130.6%40696.7%59 (100%)58 (98.3%)0/0/0/00
Protein mutations:none
Codon mutations:none
10613220.5%19197.9%27 (100%)27 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
18722114.2%19591.5%35 (100%)34 (97.1%)0/0/0/00
Protein mutations:none
Codon mutations:none
9511112.1%10090.9%17 (100%)16 (94.1%)0/0/0/00
Protein mutations:none
Codon mutations:none
244213.1%13496.4%19 (100%)18 (94.7%)0/0/0/00
Protein mutations:none
Codon mutations:none
3485438.1%43094.1%69 (100%)67 (97.1%)0/0/0/00
Protein mutations:none
Codon mutations:none
2211055.5%41892.3%61 (100%)58 (95.1%)0/0/0/02
Protein mutations:S90* (13270C>A), E99G (13297A>G)
Codon mutations:TCG90TAG (13270C>A), GAG99GGG (13297A>G)
114251.1%83096.8%119 (100%)116 (97.5%)0/0/0/00
Protein mutations:none
Codon mutations:CGA5CGG (13345A>G)
14322323.0%45192.4%57 (100%)56 (98.2%)0/0/0/00
Protein mutations:none
Codon mutations:none
9511712.0%12788.2%23 (100%)22 (95.7%)0/0/0/00
Protein mutations:none
Codon mutations:none
60103127.4%196396.5%311 (100%)306 (98.4%)0/0/0/00
Protein mutations:none
Codon mutations:none
156046.0%186797.1%296 (100%)292 (98.6%)0/0/0/00
Protein mutations:none
Codon mutations:none
47753811.5%45797.9%62 (100%)62 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
14388.4%23195.9%38 (100%)37 (97.4%)0/0/0/01
Protein mutations:none
Codon mutations:none
789317.2%10791.5%16 (100%)16 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
19864.3%50794.1%63 (100%)61 (96.8%)0/0/0/01
Protein mutations:none
Codon mutations:none
128686.0%158999.5%246 (100%)246 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
128686.0%163198.0%246 (100%)245 (99.6%)0/0/0/01
Protein mutations:none
Codon mutations:none
2927681.5%144697.8%225 (100%)224 (99.6%)0/0/0/01
Protein mutations:none
Codon mutations:none
3423461.3%129098.0%187 (100%)185 (98.9%)0/0/0/00
Protein mutations:none
Codon mutations:none
151100%325100%51 (100%)51 (100%)0/0/0/01
Protein mutations:none
Codon mutations:AAC19AAT (28568C>T), GAT44GAC (28643T>C)
138176.1%203498.3%290 (100%)287 (99.0%)0/0/0/01
Protein mutations:none
Codon mutations:none
67988.6%49497.2%70 (100%)70 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
35496.6%9596.0%15 (100%)15 (100%)0/0/0/00
Protein mutations:none
Codon mutations:none
14517110.3%20296.2%27 (100%)27 (100%)0/0/0/00
Protein mutations:none
Codon mutations:none
2012043.2%71199.4%101 (100%)100 (99.0%)0/0/0/00
Protein mutations:none
Codon mutations:none
110594.6%73199.5%105 (100%)104 (99.0%)0/0/0/00
Protein mutations:none
Codon mutations:none
10412819.5%153100%25 (100%)25 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
736179.5%192698.4%287 (100%)284 (99.0%)0/0/0/01
Protein mutations:none
Codon mutations:none
15662.5%28094.9%45 (100%)43 (95.6%)0/0/0/00
Protein mutations:none
Codon mutations:none
1621663.0%4591.8%5 (100%)5 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
13120.9%21597.3%31 (100%)30 (96.8%)0/0/0/00
Protein mutations:none
Codon mutations:none
165100%489100%65 (100%)65 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
*: Inserts / Deletes / Misaligned / Frameshifts

Analysis details

This analysis was performed with panviral2.43