Version 2.16.3
Genome Detective Platform

NGS Details (SRR3458562_Metagenome_example): Enterobacteria phage Sf101

Assembly

11035 (23 contig(s))
11.5
1129
72.85 rpm (after QC)
0
read mapping against reference + variant calling
Bcf Tools

Coverage Map

Variant Tables

Assignment

Enterobacteria phage Sf101 (Taxonomy ID: 1524881)
99.2387
99.1014
20
66

Alignment

21722.0 (NT) + 23883.0 (AA) = 45605.0
99.0918
Local, heuristic, nucleotide (BLASTN)

Alignment Detailed Statistics

BeginEndCoverageScoreConcordanceMatchesIdentitiesI/D/M/F*Stop Codons
NT3733853728.5%2172298.5%11034 (99.9%)10950 (99.2%)0/1
Mutations:674A>C, 692A>G, 704A>G, 779A>G, 839G>A, 863T>C, 869T>G, 872G>A, 1358G>A, 1424A>T, 1448C>T, 1466C>T, 1682C>T, 1691G>A, 1700A>T, 1736T>C, 1962G>A, 2118A>G, 2616T>G, 2754T>C, 2784G>A, 3171C>T, 3174C>T, 3285G>T, 3291G>A, 3303T>C, 3405T>C, 3420C>T, 3471T>C, 3513A>G, 3516A>C, 3528G>A, 3552T>C, 3642A>G, 3655A>C, 3768A>G, 3828A>G, 3843A>C, 3861T>C, 3975T>C, 4219G>A, 4251C>A, 4286C>A, 4356A>T, 4407T>C, 4560C>T, 4671A>T, 4738T>C, 4776A>G, 4779C>T, 4782C>T, 4914A>G, 4957C>T, 5048C>A, 5277T>A, 5337G>T, 5340A>G, 5361A>C, 5529A>G, 5556C>T, 6153G>A, 6193G>T, 6288C>T, 6365T>A, 6383T>C, 6726T>C, 6819T>C, 6829T>A, 7053G>T, 7074A>G, 23543G>A, 23546A>G, 32775T>A, 32857G>T, 32996C>A, 33001A>C, 33262C>A, 33341T>A, 37237A>G, 37370T>C, 37385C>T, 37453T>C, 37483delA, 38213G>A, 38358A>G
CDS
12314113.5%117100%19 (100%)19 (100%)0/0/0/01
147297.9%324899.6%462 (100%)461 (99.8%)0/0/0/01
1733100%495498.9%733 (100%)730 (99.6%)0/0/1/01
Protein mutations:D258E (2616T>G), K605Q (3655A>C)
Codon mutations:GTG40GTA (1962G>A), AGA92AGG (2118A>G), GAT258GAG (2616T>G), GGT304GGC (2754T>C), GGG314GGA (2784G>A), GTC443GTT (3171C>T), GCC444GCT (3174C>T), GCG481GCT (3285G>T), GAG483GAA (3291G>A), AGT487AGC (3303T>C), AAT521AAC (3405T>C), GGC526GGT (3420C>T), GAT543GAC (3471T>C), CCA557CCG (3513A>G), CCA558CCC (3516A>C), AAG562AAA (3528G>A), ATT570ATC (3552T>C), ACA600ACG (3642A>G), AAG605CAG (3655A>C), GAA642GAG (3768A>G), GCA662GCG (3828A>G), ATA667ATC (3843A>C), AGT673AGC (3861T>C), GGT711GGC (3975T>C)
1298100%189999.6%298 (100%)296 (99.3%)0/0/0/01
Protein mutations:E30K (4219G>A), A52D (4286C>A)
Codon mutations:GAA30AAA (4219G>A), TCC40TCA (4251C>A), GCC52GAC (4286C>A), GCA75GCT (4356A>T), CGT92CGC (4407T>C), AAC143AAT (4560C>T), GTA180GTT (4671A>T), TTG203CTG (4738T>C), GCA215GCG (4776A>G), CTC216CTT (4779C>T), GGC217GGT (4782C>T), ACA261ACG (4914A>G), CTG276TTG (4957C>T)
141886.4%240099.0%361 (100%)357 (98.9%)0/0/0/01
Protein mutations:A2D (5048C>A), A384S (6193G>T)
Codon mutations:GCT2GAT (5048C>A), TCT78TCA (5277T>A), CCG98CCT (5337G>T), GAA99GAG (5340A>G), CGA106CGC (5361A>C), GTA162GTG (5529A>G), TTC171TTT (5556C>T), ACG370ACA (6153G>A), GCC384TCC (6193G>T), GCC415GCT (6288C>T)
163100%416100%63 (100%)63 (100%)0/0/0/01
1154100%103798.2%154 (100%)153 (99.4%)0/0/0/01
Protein mutations:C108S (6829T>A)
Codon mutations:GGT73GGC (6726T>C), ACT104ACC (6819T>C), TGT108AGT (6829T>A)
135429.4%94097.4%139 (100%)137 (98.6%)0/0/0/00
13515211.8%124100%18 (100%)18 (100%)0/0/0/01
1125.2%6487.7%12 (100%)11 (91.7%)0/0/0/00
12333.8%13295.7%22 (100%)21 (95.5%)0/0/0/00
149772.7%52494.8%72 (100%)70 (97.2%)0/0/0/00
112435.2%30595.9%45 (100%)43 (95.6%)0/0/0/00
3820281.7%104799.4%165 (100%)165 (100%)0/0/0/01
67682.9%746.7%2 (100%)2 (100%)0/0/0/01
245649.3%22596.6%33 (100%)33 (100%)0/0/0/00
182737.0%7390.1%10 (100%)10 (100%)0/0/0/01
113367.3%66297.5%99 (100%)97 (98.0%)0/0/0/00
119632.1%39495.6%63 (100%)62 (98.4%)0/0/0/01
159100%42494.6%59 (100%)57 (96.6%)0/0/0/01
Protein mutations:S24T (32775T>A), W51L (32857G>T)
Codon mutations:TCC24ACC (32775T>A), TGG51TTG (32857G>T)
113775.9%78098.0%104 (100%)102 (98.1%)0/0/0/01
Protein mutations:L38I (32996C>A)
Codon mutations:CTC38ATC (32996C>A), CCA39CCC (33001A>C), GTC126GTA (33262C>A)
13661.0%23792.6%36 (100%)35 (97.2%)0/0/0/00
12437.5%17597.8%24 (100%)23 (95.8%)0/0/0/00
13015918.9%188100%30 (100%)30 (100%)0/0/0/01
114695.9%88198.7%140 (100%)139 (99.3%)0/0/0/01
162100%388100%62 (100%)62 (100%)0/0/0/01
151100%31485.8%51 (100%)49 (96.1%)0/0/1/01
Protein mutations:D22G (37237A>G)
Codon mutations:GAT22GGT (37237A>G)
1181100%1238100%181 (100%)181 (100%)0/0/0/01
125100%205100%25 (100%)25 (100%)0/0/0/01
17997.5%48598.2%79 (100%)78 (98.7%)0/0/0/00
Proteins
12314113.5%117100%19 (100%)19 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
147297.9%324899.6%462 (100%)461 (99.8%)0/0/0/01
Protein mutations:none
Codon mutations:TCA83TCC (674A>C), GGA89GGG (692A>G), GAA93GAG (704A>G), GAA118GAG (779A>G), CCG138CCA (839G>A), TTT146TTC (863T>C), CCT148CCG (869T>G), AAG149AAA (872G>A), GCG311GCA (1358G>A), GCA333GCT (1424A>T), GCC341GCT (1448C>T), GGC347GGT (1466C>T), AAC419AAT (1682C>T), AAG422AAA (1691G>A), CTA425CTT (1700A>T), ATT437ATC (1736T>C)
1733100%495498.9%733 (100%)730 (99.6%)0/0/1/01
Protein mutations:D258E (2616T>G), K605Q (3655A>C)
Codon mutations:GTG40GTA (1962G>A), AGA92AGG (2118A>G), GAT258GAG (2616T>G), GGT304GGC (2754T>C), GGG314GGA (2784G>A), GTC443GTT (3171C>T), GCC444GCT (3174C>T), GCG481GCT (3285G>T), GAG483GAA (3291G>A), AGT487AGC (3303T>C), AAT521AAC (3405T>C), GGC526GGT (3420C>T), GAT543GAC (3471T>C), CCA557CCG (3513A>G), CCA558CCC (3516A>C), AAG562AAA (3528G>A), ATT570ATC (3552T>C), ACA600ACG (3642A>G), AAG605CAG (3655A>C), GAA642GAG (3768A>G), GCA662GCG (3828A>G), ATA667ATC (3843A>C), AGT673AGC (3861T>C), GGT711GGC (3975T>C)
1298100%189999.6%298 (100%)296 (99.3%)0/0/0/01
Protein mutations:E30K (4219G>A), A52D (4286C>A)
Codon mutations:GAA30AAA (4219G>A), TCC40TCA (4251C>A), GCC52GAC (4286C>A), GCA75GCT (4356A>T), CGT92CGC (4407T>C), AAC143AAT (4560C>T), GTA180GTT (4671A>T), TTG203CTG (4738T>C), GCA215GCG (4776A>G), CTC216CTT (4779C>T), GGC217GGT (4782C>T), ACA261ACG (4914A>G), CTG276TTG (4957C>T)
141886.4%240099.0%361 (100%)357 (98.9%)0/0/0/01
Protein mutations:A2D (5048C>A), A384S (6193G>T)
Codon mutations:GCT2GAT (5048C>A), TCT78TCA (5277T>A), CCG98CCT (5337G>T), GAA99GAG (5340A>G), CGA106CGC (5361A>C), GTA162GTG (5529A>G), TTC171TTT (5556C>T), ACG370ACA (6153G>A), GCC384TCC (6193G>T), GCC415GCT (6288C>T)
163100%416100%63 (100%)63 (100%)0/0/0/01
Protein mutations:none
Codon mutations:CCT9CCA (6365T>A), TGT15TGC (6383T>C)
1154100%103798.2%154 (100%)153 (99.4%)0/0/0/01
Protein mutations:C108S (6829T>A)
Codon mutations:GGT73GGC (6726T>C), ACT104ACC (6819T>C), TGT108AGT (6829T>A)
135429.4%94097.4%139 (100%)137 (98.6%)0/0/0/00
Protein mutations:none
Codon mutations:CTG25CTT (7053G>T), ACA32ACG (7074A>G)
13515211.8%124100%18 (100%)18 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
1125.2%6487.7%12 (100%)11 (91.7%)0/0/0/00
Protein mutations:none
Codon mutations:none
12333.8%13295.7%22 (100%)21 (95.5%)0/0/0/00
Protein mutations:none
Codon mutations:none
149772.7%52494.8%72 (100%)70 (97.2%)0/0/0/00
Protein mutations:none
Codon mutations:none
112435.2%30595.9%45 (100%)43 (95.6%)0/0/0/00
Protein mutations:none
Codon mutations:none
3820281.7%104799.4%165 (100%)165 (100%)0/0/0/01
Protein mutations:none
Codon mutations:AAT46AAC (23546A>G), GGC47GGT (23543G>A)
67682.9%746.7%2 (100%)2 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
245649.3%22596.6%33 (100%)33 (100%)0/0/0/00
Protein mutations:none
Codon mutations:none
182737.0%7390.1%10 (100%)10 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
113367.3%66297.5%99 (100%)97 (98.0%)0/0/0/00
Protein mutations:none
Codon mutations:none
119632.1%39495.6%63 (100%)62 (98.4%)0/0/0/01
Protein mutations:none
Codon mutations:none
159100%42494.6%59 (100%)57 (96.6%)0/0/0/01
Protein mutations:S24T (32775T>A), W51L (32857G>T)
Codon mutations:TCC24ACC (32775T>A), TGG51TTG (32857G>T)
113775.9%78098.0%104 (100%)102 (98.1%)0/0/0/01
Protein mutations:L38I (32996C>A)
Codon mutations:CTC38ATC (32996C>A), CCA39CCC (33001A>C), GTC126GTA (33262C>A)
13661.0%23792.6%36 (100%)35 (97.2%)0/0/0/00
Protein mutations:none
Codon mutations:GCT18GCA (33341T>A)
12437.5%17597.8%24 (100%)23 (95.8%)0/0/0/00
Protein mutations:none
Codon mutations:none
13015918.9%188100%30 (100%)30 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
114695.9%88198.7%140 (100%)139 (99.3%)0/0/0/01
Protein mutations:none
Codon mutations:none
162100%388100%62 (100%)62 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
151100%31485.8%51 (100%)49 (96.1%)0/0/1/01
Protein mutations:D22G (37237A>G)
Codon mutations:GAT22GGT (37237A>G)
1181100%1238100%181 (100%)181 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
125100%205100%25 (100%)25 (100%)0/0/0/01
Protein mutations:none
Codon mutations:AGG9AGA (38213G>A)
17997.5%48598.2%79 (100%)78 (98.7%)0/0/0/00
Protein mutations:none
Codon mutations:AAA19AAG (38358A>G)
*: Inserts / Deletes / Misaligned / Frameshifts

Analysis details

This analysis was performed with panviral2.43