Version 2.22.0
Genome Detective Platform

NGS Details (SRR3458562_Metagenome_example): Enterobacteria phage Sf101

Assembly

11035 (23 contig(s))
11.5
1129
72.85 rpm (after QC)
0
read mapping against reference + variant calling
Bcf Tools

Coverage Map

Variant Tables

Assignment

Enterobacteria phage Sf101 (Taxonomy ID: 1524881)
99.2387
99.1014
20
66

Alignment

21722.0 (NT) + 23883.0 (AA) = 45605.0
99.0918
Local, heuristic, nucleotide (BLASTN)

Alignment Detailed Statistics

BeginEndCoverageScoreConcordanceMatchesIdentitiesI/D/M/F*Stop Codons
NT3733853728.5%2172298.5%11034 (99.9%)10950 (99.2%)0/1
Mutations:
674A>C 692A>G 704A>G 779A>G 839G>A 863T>C 869T>G 872G>A 1358G>A 1424A>T 1448C>T 1466C>T 1682C>T 1691G>A 1700A>T 1736T>C 1962G>A 2118A>G 2616T>G 2754T>C 2784G>A 3171C>T 3174C>T 3285G>T 3291G>A 3303T>C 3405T>C 3420C>T 3471T>C 3513A>G 3516A>C 3528G>A 3552T>C 3642A>G 3655A>C 3768A>G 3828A>G 3843A>C 3861T>C 3975T>C 4219G>A 4251C>A 4286C>A 4356A>T 4407T>C 4560C>T 4671A>T 4738T>C 4776A>G 4779C>T 4782C>T 4914A>G 4957C>T 5048C>A 5277T>A 5337G>T 5340A>G 5361A>C 5529A>G 5556C>T 6153G>A 6193G>T 6288C>T 6365T>A 6383T>C 6726T>C 6819T>C 6829T>A 7053G>T 7074A>G 23543G>A 23546A>G 32775T>A 32857G>T 32996C>A 33001A>C 33262C>A 33341T>A 37237A>G 37370T>C 37385C>T 37453T>C 37483delA 38213G>A 38358A>G
CDS
12314113.5%117100%19 (100%)19 (100%)0/0/0/01
147297.9%324899.6%462 (100%)461 (99.8%)0/0/0/01
1733100%495498.9%733 (100%)730 (99.6%)0/0/1/01
Protein mutations:
D258E (2616T>G) K605Q (3655A>C)
Codon mutations:
GTG40GTA (1962G>A) AGA92AGG (2118A>G) GAT258GAG (2616T>G) GGT304GGC (2754T>C) GGG314GGA (2784G>A) GTC443GTT (3171C>T) GCC444GCT (3174C>T) GCG481GCT (3285G>T) GAG483GAA (3291G>A) AGT487AGC (3303T>C) AAT521AAC (3405T>C) GGC526GGT (3420C>T) GAT543GAC (3471T>C) CCA557CCG (3513A>G) CCA558CCC (3516A>C) AAG562AAA (3528G>A) ATT570ATC (3552T>C) ACA600ACG (3642A>G) AAG605CAG (3655A>C) GAA642GAG (3768A>G) GCA662GCG (3828A>G) ATA667ATC (3843A>C) AGT673AGC (3861T>C) GGT711GGC (3975T>C)
1298100%189999.6%298 (100%)296 (99.3%)0/0/0/01
Protein mutations:
E30K (4219G>A) A52D (4286C>A)
Codon mutations:
GAA30AAA (4219G>A) TCC40TCA (4251C>A) GCC52GAC (4286C>A) GCA75GCT (4356A>T) CGT92CGC (4407T>C) AAC143AAT (4560C>T) GTA180GTT (4671A>T) TTG203CTG (4738T>C) GCA215GCG (4776A>G) CTC216CTT (4779C>T) GGC217GGT (4782C>T) ACA261ACG (4914A>G) CTG276TTG (4957C>T)
141886.4%240099.0%361 (100%)357 (98.9%)0/0/0/01
Protein mutations:
A2D (5048C>A) A384S (6193G>T)
Codon mutations:
GCT2GAT (5048C>A) TCT78TCA (5277T>A) CCG98CCT (5337G>T) GAA99GAG (5340A>G) CGA106CGC (5361A>C) GTA162GTG (5529A>G) TTC171TTT (5556C>T) ACG370ACA (6153G>A) GCC384TCC (6193G>T) GCC415GCT (6288C>T)
163100%416100%63 (100%)63 (100%)0/0/0/01
1154100%103798.2%154 (100%)153 (99.4%)0/0/0/01
Protein mutations:
C108S (6829T>A)
Codon mutations:
GGT73GGC (6726T>C) ACT104ACC (6819T>C) TGT108AGT (6829T>A)
135429.4%94097.4%139 (100%)137 (98.6%)0/0/0/00
13515211.8%124100%18 (100%)18 (100%)0/0/0/01
1125.2%6487.7%12 (100%)11 (91.7%)0/0/0/00
12333.8%13295.7%22 (100%)21 (95.5%)0/0/0/00
149772.7%52494.8%72 (100%)70 (97.2%)0/0/0/00
112435.2%30595.9%45 (100%)43 (95.6%)0/0/0/00
3820281.7%104799.4%165 (100%)165 (100%)0/0/0/01
67682.9%746.7%2 (100%)2 (100%)0/0/0/01
245649.3%22596.6%33 (100%)33 (100%)0/0/0/00
182737.0%7390.1%10 (100%)10 (100%)0/0/0/01
113367.3%66297.5%99 (100%)97 (98.0%)0/0/0/00
119632.1%39495.6%63 (100%)62 (98.4%)0/0/0/01
159100%42494.6%59 (100%)57 (96.6%)0/0/0/01
Protein mutations:
S24T (32775T>A) W51L (32857G>T)
Codon mutations:
TCC24ACC (32775T>A) TGG51TTG (32857G>T)
113775.9%78098.0%104 (100%)102 (98.1%)0/0/0/01
Protein mutations:
L38I (32996C>A)
Codon mutations:
CTC38ATC (32996C>A) CCA39CCC (33001A>C) GTC126GTA (33262C>A)
13661.0%23792.6%36 (100%)35 (97.2%)0/0/0/00
12437.5%17597.8%24 (100%)23 (95.8%)0/0/0/00
13015918.9%188100%30 (100%)30 (100%)0/0/0/01
114695.9%88198.7%140 (100%)139 (99.3%)0/0/0/01
162100%388100%62 (100%)62 (100%)0/0/0/01
151100%31485.8%51 (100%)49 (96.1%)0/0/1/01
Protein mutations:
D22G (37237A>G)
Codon mutations:
GAT22GGT (37237A>G)
1181100%1238100%181 (100%)181 (100%)0/0/0/01
125100%205100%25 (100%)25 (100%)0/0/0/01
17997.5%48598.2%79 (100%)78 (98.7%)0/0/0/00
Proteins
12314113.5%117100%19 (100%)19 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
147297.9%324899.6%462 (100%)461 (99.8%)0/0/0/01
Protein mutations:none
Codon mutations:
TCA83TCC (674A>C) GGA89GGG (692A>G) GAA93GAG (704A>G) GAA118GAG (779A>G) CCG138CCA (839G>A) TTT146TTC (863T>C) CCT148CCG (869T>G) AAG149AAA (872G>A) GCG311GCA (1358G>A) GCA333GCT (1424A>T) GCC341GCT (1448C>T) GGC347GGT (1466C>T) AAC419AAT (1682C>T) AAG422AAA (1691G>A) CTA425CTT (1700A>T) ATT437ATC (1736T>C)
1733100%495498.9%733 (100%)730 (99.6%)0/0/1/01
Protein mutations:
D258E (2616T>G) K605Q (3655A>C)
Codon mutations:
GTG40GTA (1962G>A) AGA92AGG (2118A>G) GAT258GAG (2616T>G) GGT304GGC (2754T>C) GGG314GGA (2784G>A) GTC443GTT (3171C>T) GCC444GCT (3174C>T) GCG481GCT (3285G>T) GAG483GAA (3291G>A) AGT487AGC (3303T>C) AAT521AAC (3405T>C) GGC526GGT (3420C>T) GAT543GAC (3471T>C) CCA557CCG (3513A>G) CCA558CCC (3516A>C) AAG562AAA (3528G>A) ATT570ATC (3552T>C) ACA600ACG (3642A>G) AAG605CAG (3655A>C) GAA642GAG (3768A>G) GCA662GCG (3828A>G) ATA667ATC (3843A>C) AGT673AGC (3861T>C) GGT711GGC (3975T>C)
1298100%189999.6%298 (100%)296 (99.3%)0/0/0/01
Protein mutations:
E30K (4219G>A) A52D (4286C>A)
Codon mutations:
GAA30AAA (4219G>A) TCC40TCA (4251C>A) GCC52GAC (4286C>A) GCA75GCT (4356A>T) CGT92CGC (4407T>C) AAC143AAT (4560C>T) GTA180GTT (4671A>T) TTG203CTG (4738T>C) GCA215GCG (4776A>G) CTC216CTT (4779C>T) GGC217GGT (4782C>T) ACA261ACG (4914A>G) CTG276TTG (4957C>T)
141886.4%240099.0%361 (100%)357 (98.9%)0/0/0/01
Protein mutations:
A2D (5048C>A) A384S (6193G>T)
Codon mutations:
GCT2GAT (5048C>A) TCT78TCA (5277T>A) CCG98CCT (5337G>T) GAA99GAG (5340A>G) CGA106CGC (5361A>C) GTA162GTG (5529A>G) TTC171TTT (5556C>T) ACG370ACA (6153G>A) GCC384TCC (6193G>T) GCC415GCT (6288C>T)
163100%416100%63 (100%)63 (100%)0/0/0/01
Protein mutations:none
Codon mutations:
CCT9CCA (6365T>A) TGT15TGC (6383T>C)
1154100%103798.2%154 (100%)153 (99.4%)0/0/0/01
Protein mutations:
C108S (6829T>A)
Codon mutations:
GGT73GGC (6726T>C) ACT104ACC (6819T>C) TGT108AGT (6829T>A)
135429.4%94097.4%139 (100%)137 (98.6%)0/0/0/00
Protein mutations:none
Codon mutations:
CTG25CTT (7053G>T) ACA32ACG (7074A>G)
13515211.8%124100%18 (100%)18 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
1125.2%6487.7%12 (100%)11 (91.7%)0/0/0/00
Protein mutations:none
Codon mutations:none
12333.8%13295.7%22 (100%)21 (95.5%)0/0/0/00
Protein mutations:none
Codon mutations:none
149772.7%52494.8%72 (100%)70 (97.2%)0/0/0/00
Protein mutations:none
Codon mutations:none
112435.2%30595.9%45 (100%)43 (95.6%)0/0/0/00
Protein mutations:none
Codon mutations:none
3820281.7%104799.4%165 (100%)165 (100%)0/0/0/01
Protein mutations:none
Codon mutations:
AAT46AAC (23546A>G) GGC47GGT (23543G>A)
67682.9%746.7%2 (100%)2 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
245649.3%22596.6%33 (100%)33 (100%)0/0/0/00
Protein mutations:none
Codon mutations:none
182737.0%7390.1%10 (100%)10 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
113367.3%66297.5%99 (100%)97 (98.0%)0/0/0/00
Protein mutations:none
Codon mutations:none
119632.1%39495.6%63 (100%)62 (98.4%)0/0/0/01
Protein mutations:none
Codon mutations:none
159100%42494.6%59 (100%)57 (96.6%)0/0/0/01
Protein mutations:
S24T (32775T>A) W51L (32857G>T)
Codon mutations:
TCC24ACC (32775T>A) TGG51TTG (32857G>T)
113775.9%78098.0%104 (100%)102 (98.1%)0/0/0/01
Protein mutations:
L38I (32996C>A)
Codon mutations:
CTC38ATC (32996C>A) CCA39CCC (33001A>C) GTC126GTA (33262C>A)
13661.0%23792.6%36 (100%)35 (97.2%)0/0/0/00
Protein mutations:none
Codon mutations:
GCT18GCA (33341T>A)
12437.5%17597.8%24 (100%)23 (95.8%)0/0/0/00
Protein mutations:none
Codon mutations:none
13015918.9%188100%30 (100%)30 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
114695.9%88198.7%140 (100%)139 (99.3%)0/0/0/01
Protein mutations:none
Codon mutations:none
162100%388100%62 (100%)62 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
151100%31485.8%51 (100%)49 (96.1%)0/0/1/01
Protein mutations:
D22G (37237A>G)
Codon mutations:
GAT22GGT (37237A>G)
1181100%1238100%181 (100%)181 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
125100%205100%25 (100%)25 (100%)0/0/0/01
Protein mutations:none
Codon mutations:
AGG9AGA (38213G>A)
17997.5%48598.2%79 (100%)78 (98.7%)0/0/0/00
Protein mutations:none
Codon mutations:
AAA19AAG (38358A>G)
*: Inserts / Deletes / Misaligned / Frameshifts

Analysis details

This analysis was performed with panviral2.43