NGS Details (SRR3458562_Metagenome_example): Sinsheimervirus phiX174

Assembly

1841 (9 contig(s))
1.2
17
1.10 rpm (after QC)
0
de novo + reference guided assembly
Bcf Tools

Coverage Map

Variant Tables

Assignment

Sinsheimervirus phiX174 (Taxonomy ID: 10847)
98.5877
95.8025
5
11

Alignment

3578.0 (NT) + 5217.0 (AA) = 8795.0
97.3221
Global, seeded, nucleotide + amino acids (AGA)

Alignment Detailed Statistics

BeginEndCoverageScoreConcordanceMatchesIdentitiesI/D/M/F*Stop Codons
NT751538634.2%357897.2%1841 (100%)1815 (98.6%)0/0
Mutations:833G>A, 934T>G, 953C>A, 1600T>A, 1624A>C, 2932T>G, 2934T>C, 2936T>G, 2938G>C, 2940G>A, 2942T>G, 2946G>A, 2948T>A, 2951C>G, 2967C>G, 2972T>C, 2981C>A, 2984G>A, 2985T>A, 3028T>C, 3133C>T, 3814T>G, 4277A>T, 4964A>C, 5017A>C, 5344T>G
CDS
146943.2%141995.9%222 (100%)215 (96.8%)0/0/0/00
Protein mutations:K328N (4964A>C), K346T (5017A>C), F455C (5344T>G)
Codon mutations:GCA99GCT (4277A>T), AAA328AAC (4964A>C), AAA346ACA (5017A>C), TTT455TGT (5344T>G)
13729735.7%73593.3%122 (100%)116 (95.1%)0/0/0/00
Protein mutations:K156N (4964A>C), K174T (5017A>C), F283C (5344T>G)
Codon mutations:AAA156AAC (4964A>C), AAA174ACA (5017A>C), TTT283TGT (5344T>G)
210462.8%54695.8%76 (100%)74 (97.4%)0/0/0/00
Protein mutations:I90M (5344T>G)
Codon mutations:ATT90ATG (5344T>G)
12215320.9%19295.0%32 (100%)32 (100%)0/0/0/01
629233.7%19697.5%31 (100%)30 (96.8%)0/0/0/01
Protein mutations:R89Q (833G>A)
Codon mutations:CGG89CAG (833G>A)
139100%26597.1%39 (100%)38 (97.4%)0/0/0/01
Protein mutations:Q36K (953C>A)
Codon mutations:CGT29CGG (934T>G), CAA36AAA (953C>A)
14038339.5%118496.3%169 (100%)165 (97.6%)0/0/0/01
Protein mutations:Y200* (1600T>A), E208D (1624A>C)
Codon mutations:TAT200TAA (1600T>A), GAA208GAC (1624A>C)
132936.2%68089.8%119 (100%)106 (89.1%)0/0/0/01
Protein mutations:M1R (2932T>G), F2L (2934T>C 2936T>G), G3A (2938G>C), A4T (2940G>A 2942T>G), A6T (2946G>A 2948T>A), L13V (2967C>G), M18I (2984G>A), S19T (2985T>A), I33T (3028T>C), A68V (3133C>T), V295G (3814T>G)
Codon mutations:ATG1AGG (2932T>G), TTT2CTG (2934T>C 2936T>G), GGT3GCT (2938G>C), GCT4ACG (2940G>A 2942T>G), GCT6ACA (2946G>A 2948T>A), GGC7GGG (2951C>G), CTT13GTT (2967C>G), GCT14GCC (2972T>C), GCC17GCA (2981C>A), ATG18ATA (2984G>A), TCT19ACT (2985T>A), ATT33ACT (3028T>C), GCC68GTC (3133C>T), GTT295GGT (3814T>G)
Proteins
146943.2%141995.9%222 (100%)215 (96.8%)0/0/0/00
Protein mutations:K328N (4964A>C), K346T (5017A>C), F455C (5344T>G)
Codon mutations:GCA99GCT (4277A>T), AAA328AAC (4964A>C), AAA346ACA (5017A>C), TTT455TGT (5344T>G)
13729735.7%73593.3%122 (100%)116 (95.1%)0/0/0/00
Protein mutations:K156N (4964A>C), K174T (5017A>C), F283C (5344T>G)
Codon mutations:AAA156AAC (4964A>C), AAA174ACA (5017A>C), TTT283TGT (5344T>G)
210462.8%54695.8%76 (100%)74 (97.4%)0/0/0/00
Protein mutations:I90M (5344T>G)
Codon mutations:ATT90ATG (5344T>G)
12215320.9%19295.0%32 (100%)32 (100%)0/0/0/01
Protein mutations:none
Codon mutations:GCG148GCA (833G>A)
629233.7%19697.5%31 (100%)30 (96.8%)0/0/0/01
Protein mutations:R89Q (833G>A)
Codon mutations:CGG89CAG (833G>A)
139100%26597.1%39 (100%)38 (97.4%)0/0/0/01
Protein mutations:Q36K (953C>A)
Codon mutations:CGT29CGG (934T>G), CAA36AAA (953C>A)
14038339.5%118496.3%169 (100%)165 (97.6%)0/0/0/01
Protein mutations:Y200* (1600T>A), E208D (1624A>C)
Codon mutations:TAT200TAA (1600T>A), GAA208GAC (1624A>C)
132936.2%68089.8%119 (100%)106 (89.1%)0/0/0/01
Protein mutations:M1R (2932T>G), F2L (2934T>C 2936T>G), G3A (2938G>C), A4T (2940G>A 2942T>G), A6T (2946G>A 2948T>A), L13V (2967C>G), M18I (2984G>A), S19T (2985T>A), I33T (3028T>C), A68V (3133C>T), V295G (3814T>G)
Codon mutations:ATG1AGG (2932T>G), TTT2CTG (2934T>C 2936T>G), GGT3GCT (2938G>C), GCT4ACG (2940G>A 2942T>G), GCT6ACA (2946G>A 2948T>A), GGC7GGG (2951C>G), CTT13GTT (2967C>G), GCT14GCC (2972T>C), GCC17GCA (2981C>A), ATG18ATA (2984G>A), TCT19ACT (2985T>A), ATT33ACT (3028T>C), GCC68GTC (3133C>T), GTT295GGT (3814T>G)
*: Inserts / Deletes / Misaligned / Frameshifts

Analysis details

This analysis was performed with panviral2.43