NGS Details (SRR3458562_Metagenome_example): Peduovirus P24E6b

Assembly

1064 (5 contig(s))
24.2
236
15.23 rpm (after QC)
0
de novo + reference guided assembly
Bcf Tools

Coverage Map

Variant Tables

Assignment

Peduovirus P24E6b (Taxonomy ID: 2844222)
89.8496
90.9348
0
44

Alignment

1696.0 (NT) + 2158.0 (AA) = 3854.0
86.2578
Local, heuristic, nucleotide (BLASTN)

Alignment Detailed Statistics

BeginEndCoverageScoreConcordanceMatchesIdentitiesI/D/M/F*Stop Codons
NT12549165843.4%169679.7%1064 (100%)956 (89.8%)0/0
Mutations:12589C>G, 12616C>T, 12654A>G, 12681T>C, 12687G>T, 12721G>A, 12725C>G, 12741T>C, 12752T>A, 12759G>A, 12760A>C, 12762A>T, 12768C>A, 12772G>T, 12774A>G, 12777C>T, 12780G>C, 12783T>C, 12790G>A, 12791C>T, 12792C>T, 12793A>G, 12794A>G, 12795T>A, 12797G>C, 12798G>A, 12807C>T, 12809C>A, 12816T>A, 12819G>A, 12825C>A, 12827T>C, 12834T>C, 12837C>T, 12838A>G, 12839C>T, 12843A>G, 12844T>G, 12848C>T, 12849T>A, 12852C>G, 12855C>A, 12856C>G, 12859T>C, 12861G>A, 12870T>C, 12873A>T, 12879G>T, 12882T>C, 12883A>T, 12885G>T, 12888T>C, 12891A>T, 12893C>G, 12897C>A, 12900G>A, 12902A>C, 12904C>T, 12906T>A, 12910T>C, 12916C>A, 12918T>A, 12924T>C, 12927C>T, 12933T>A, 12939T>C, 12942A>T, 12944A>T, 12951C>A, 12952A>C, 12957T>C, 13381G>C, 13383A>G, 13384G>A, 13386A>T, 13395C>A, 13396T>A, 13397T>A, 13398T>C, 13401G>T, 13407T>G, 13408C>T, 13410G>A, 13411A>G, 13413T>G, 13414C>T, 13419C>T, 13443A>C, 13444T>A, 13449T>C, 13464C>A, 13497G>T, 13503T>G, 13518C>T, 13570G>T, 13575T>C, 13593C>T, 13597A>T, 13602G>A, 13641G>C, 13728T>C, 13864T>C, 13938G>A, 16457C>A, 16459G>A, 16478G>A, 16519A>T, 16562G>T
CDS
23068233.5%192890.0%308 (100%)280 (90.9%)0/0/1/00
Protein mutations:L243V (12589C>G), P252S (12616C>T), A287T (12721G>A), A288G (12725C>G), F297Y (12752T>A), V304L (12772G>T 12774A>G), M306I (12780G>C), A310I (12790G>A 12791C>T 12792C>T), N311G (12793A>G 12794A>G 12795T>A), R312T (12797G>C 12798G>A), T316N (12809C>A), V322A (12827T>C), T326V (12838A>G 12839C>T), S328A (12844T>G), A329V (12848C>T 12849T>A), P332A (12856C>G), T341S (12883A>T 12885G>T), A344G (12893C>G), H347P (12902A>C), Y361F (12944A>T), N364H (12952A>C), F512N (13396T>A 13397T>A 13398T>C), N517E (13411A>G 13413T>G), S528T (13444T>A), A570S (13570G>T), T579S (13597A>T)
Codon mutations:CTT243GTT (12589C>G), CCT252TCT (12616C>T), ACA264ACG (12654A>G), AAT273AAC (12681T>C), CCG275CCT (12687G>T), GCT287ACT (12721G>A), GCC288GGC (12725C>G), GGT293GGC (12741T>C), TTT297TAT (12752T>A), ACG299ACA (12759G>A), AGA300CGT (12760A>C 12762A>T), GGC302GGA (12768C>A), GTA304TTG (12772G>T 12774A>G), TAC305TAT (12777C>T), ATG306ATC (12780G>C), CGT307CGC (12783T>C), GCC310ATT (12790G>A 12791C>T 12792C>T), AAT311GGA (12793A>G 12794A>G 12795T>A), AGG312ACA (12797G>C 12798G>A), GGC315GGT (12807C>T), ACT316AAT (12809C>A), GGT318GGA (12816T>A), CCG319CCA (12819G>A), GGC321GGA (12825C>A), GTA322GCA (12827T>C), CGT324CGC (12834T>C), CAC325CAT (12837C>T), ACT326GTT (12838A>G 12839C>T), CAA327CAG (12843A>G), TCT328GCT (12844T>G), GCT329GTA (12848C>T 12849T>A), ACC330ACG (12852C>G), CGC331CGA (12855C>A), CCT332GCT (12856C>G), TTG333CTA (12859T>C 12861G>A), ACT336ACC (12870T>C), ATA337ATT (12873A>T), CTG339CTT (12879G>T), AAT340AAC (12882T>C), ACG341TCT (12883A>T 12885G>T), CTT342CTC (12888T>C), GGA343GGT (12891A>T), GCC344GGC (12893C>G), GCC345GCA (12897C>A), GAG346GAA (12900G>A), CAT347CCT (12902A>C), CTT348TTA (12904C>T 12906T>A), TTA350CTA (12910T>C), CGT352AGA (12916C>A 12918T>A), AGT354AGC (12924T>C), AGC355AGT (12927C>T), GCT357GCA (12933T>A), GCT359GCC (12939T>C), TCA360TCT (12942A>T), TAT361TTT (12944A>T), CGC363CGA (12951C>A), AAT364CAT (12952A>C), TAT365TAC (12957T>C), GAA507C.G (13381G>C 13383A>G), GTA508A.T (13384G>A 13386A>T), TCC511..A (13395C>A), TTT512AAC (13396T>A 13397T>A 13398T>C), TCG513.CT (13401G>T), CCT515CCG (13407T>G), CTG516TTA (13408C>T 13410G>A), AAT517GAG (13411A>G 13413T>G), CTG518TTG (13414C>T), GGC519GGT (13419C>T), CTA527CTC (13443A>C), TCC528ACC (13444T>A), AGT529AGC (13449T>C), ACC534ACA (13464C>A), GTG545GTT (13497G>T), CTT547CTG (13503T>G), AAC552AAT (13518C>T), GCA570TCA (13570G>T), GTT571GTC (13575T>C), TCC577TCT (13593C>T), ACG579TCG (13597A>T), GGG580GGA (13602G>A), GCG593GCC (13641G>C), GCT622GCC (13728T>C), TTG668CTG (13864T>C), GGG692..A (13938G>A)
529611.3%23085.8%45 (100%)41 (91.1%)0/0/0/00
Protein mutations:Q54K (16457C>A 16459G>A), G61S (16478G>A), G89C (16562G>T)
Codon mutations:CAG54AAA (16457C>A 16459G>A), GGT61AGT (16478G>A), GCA74GCT (16519A>T), GGC89TGC (16562G>T)
Proteins
23068233.5%192890.0%308 (100%)280 (90.9%)0/0/1/00
Protein mutations:L243V (12589C>G), P252S (12616C>T), A287T (12721G>A), A288G (12725C>G), F297Y (12752T>A), V304L (12772G>T 12774A>G), M306I (12780G>C), A310I (12790G>A 12791C>T 12792C>T), N311G (12793A>G 12794A>G 12795T>A), R312T (12797G>C 12798G>A), T316N (12809C>A), V322A (12827T>C), T326V (12838A>G 12839C>T), S328A (12844T>G), A329V (12848C>T 12849T>A), P332A (12856C>G), T341S (12883A>T 12885G>T), A344G (12893C>G), H347P (12902A>C), Y361F (12944A>T), N364H (12952A>C), F512N (13396T>A 13397T>A 13398T>C), N517E (13411A>G 13413T>G), S528T (13444T>A), A570S (13570G>T), T579S (13597A>T)
Codon mutations:CTT243GTT (12589C>G), CCT252TCT (12616C>T), ACA264ACG (12654A>G), AAT273AAC (12681T>C), CCG275CCT (12687G>T), GCT287ACT (12721G>A), GCC288GGC (12725C>G), GGT293GGC (12741T>C), TTT297TAT (12752T>A), ACG299ACA (12759G>A), AGA300CGT (12760A>C 12762A>T), GGC302GGA (12768C>A), GTA304TTG (12772G>T 12774A>G), TAC305TAT (12777C>T), ATG306ATC (12780G>C), CGT307CGC (12783T>C), GCC310ATT (12790G>A 12791C>T 12792C>T), AAT311GGA (12793A>G 12794A>G 12795T>A), AGG312ACA (12797G>C 12798G>A), GGC315GGT (12807C>T), ACT316AAT (12809C>A), GGT318GGA (12816T>A), CCG319CCA (12819G>A), GGC321GGA (12825C>A), GTA322GCA (12827T>C), CGT324CGC (12834T>C), CAC325CAT (12837C>T), ACT326GTT (12838A>G 12839C>T), CAA327CAG (12843A>G), TCT328GCT (12844T>G), GCT329GTA (12848C>T 12849T>A), ACC330ACG (12852C>G), CGC331CGA (12855C>A), CCT332GCT (12856C>G), TTG333CTA (12859T>C 12861G>A), ACT336ACC (12870T>C), ATA337ATT (12873A>T), CTG339CTT (12879G>T), AAT340AAC (12882T>C), ACG341TCT (12883A>T 12885G>T), CTT342CTC (12888T>C), GGA343GGT (12891A>T), GCC344GGC (12893C>G), GCC345GCA (12897C>A), GAG346GAA (12900G>A), CAT347CCT (12902A>C), CTT348TTA (12904C>T 12906T>A), TTA350CTA (12910T>C), CGT352AGA (12916C>A 12918T>A), AGT354AGC (12924T>C), AGC355AGT (12927C>T), GCT357GCA (12933T>A), GCT359GCC (12939T>C), TCA360TCT (12942A>T), TAT361TTT (12944A>T), CGC363CGA (12951C>A), AAT364CAT (12952A>C), TAT365TAC (12957T>C), GAA507C.G (13381G>C 13383A>G), GTA508A.T (13384G>A 13386A>T), TCC511..A (13395C>A), TTT512AAC (13396T>A 13397T>A 13398T>C), TCG513.CT (13401G>T), CCT515CCG (13407T>G), CTG516TTA (13408C>T 13410G>A), AAT517GAG (13411A>G 13413T>G), CTG518TTG (13414C>T), GGC519GGT (13419C>T), CTA527CTC (13443A>C), TCC528ACC (13444T>A), AGT529AGC (13449T>C), ACC534ACA (13464C>A), GTG545GTT (13497G>T), CTT547CTG (13503T>G), AAC552AAT (13518C>T), GCA570TCA (13570G>T), GTT571GTC (13575T>C), TCC577TCT (13593C>T), ACG579TCG (13597A>T), GGG580GGA (13602G>A), GCG593GCC (13641G>C), GCT622GCC (13728T>C), TTG668CTG (13864T>C), GGG692..A (13938G>A)
529611.3%23085.8%45 (100%)41 (91.1%)0/0/0/00
Protein mutations:Q54K (16457C>A 16459G>A), G61S (16478G>A), G89C (16562G>T)
Codon mutations:CAG54AAA (16457C>A 16459G>A), GGT61AGT (16478G>A), GCA74GCT (16519A>T), GGC89TGC (16562G>T)
*: Inserts / Deletes / Misaligned / Frameshifts

Analysis details

This analysis was performed with panviral2.43