NGS Details (SRR3458562_Metagenome_example): Peduovirus P22H1

Assembly

1295 (4 contig(s))
15.3
196
12.65 rpm (after QC)
1
de novo + reference guided assembly
Bcf Tools

Coverage Map

Variant Tables

Assignment

Peduovirus P22H1 (Taxonomy ID: 2844217)
91.5644
91.2935
0
45

Alignment

2170.0 (NT) + 2601.0 (AA) = 4771.0
88.401
Local, heuristic, nucleotide (BLASTN)

Alignment Detailed Statistics

BeginEndCoverageScoreConcordanceMatchesIdentitiesI/D/M/F*Stop Codons
NT564272694.0%217083.8%1295 (99.3%)1194 (91.6%)9/0
Mutations:595A>G, 637T>C, 643A>G, 772G>A, 784G>A, 786C>G, 13317G>C, 13318T>A, 13320G>C, 13326G>C, 13327T>A, 13328T>A, 13329T>G, 13338T>C, 13339A>T, 13344A>T, 13346G>T, 13350G>A, 13356T>C, 13359A>G, 13362T>C, 13363G>A, 13365C>A, 13371A>C, 13372G>A, 13374T>C, 13375A>G, 13378A>G, 13380C>T, 13381C>A, 13382T>C, 13383T>A, 13388G>A, 13392C>T, 13393A>T, 13394C>T, 13395C>A, 13397T>A, 13404C>A, 13407C>G, 13411A>C, 13412C>T, 13413C>T, 13416C>T, 13417A>G, 13422_13423insTTTAACGCG, 13426A>G, 13428C>G, 13431A>G, 13437G>A, 13438G>C, 13440A>G, 13441G>A, 13443C>T, 13446C>T, 13448C>A, 13452G>A, 13453T>A, 13455T>C, 13458A>T, 13459C>T, 13461T>G, 13464C>G, 13465C>T, 13467G>A, 13468A>G, 13470T>G, 13471C>T, 13482C>T, 13485T>C, 13488T>A, 13497G>A, 13521C>A, 13555A>C, 13566T>C, 13627G>T, 13632T>C, 13650C>T, 13654A>T, 13659G>A, 13698G>C, 13707A>C, 13755A>G, 13756G>A, 13764A>T, 13767A>G, 13773C>T, 13776T>C, 16143G>A, 16147C>T, 16149T>C, 16151T>C, 16163C>T, 16169A>G, 16177A>G, 16179T>A, 16202T>G, 16219T>A, 16302C>T, 16329C>T, 27119C>M, 27132C>T
CDS
13222828.1%69198.4%97 (100%)95 (97.9%)0/0/0/00
Protein mutations:A154P (784G>A 786C>G)
Codon mutations:GCC154CCT (784G>A 786C>G), GGC158GGT (772G>A), AAT201AAC (643A>G), GCA203GCG (637T>C), GAT217GAC (595A>G)
46262417.7%99585.9%163 (98.2%)139 (83.7%)3/0/0/00
Protein mutations:L467I (13318T>A 13320G>C), F470K (13327T>A 13328T>A 13329T>G), N474Y (13339A>T), W476L (13346G>T), A482T (13363G>A 13365C>A), G485S (13372G>A 13374T>C), T486A (13375A>G), T487A (13378A>G 13380C>T), L488T (13381C>A 13382T>C 13383T>A), G490D (13388G>A), T492L (13393A>T 13394C>T 13395C>A), F493Y (13397T>A), T498L (13411A>C 13412C>T 13413C>T), S500G (13417A>G), S501_W502insFNA (13422_13423insTTTAACGCG), S503G (13426A>G 13428C>G), E507Q (13438G>C 13440A>G), V508I (13441G>A 13443C>T), T510N (13448C>A), Y512N (13453T>A 13455T>C), N517E (13468A>G 13470T>G), A570S (13627G>T), T579S (13654A>T), A613T (13756G>A)
Codon mutations:GTG466GTC (13317G>C), TTG467ATC (13318T>A 13320G>C), CGG469CGC (13326G>C), TTT470AAG (13327T>A 13328T>A 13329T>G), GTT473GTC (13338T>C), AAT474TAT (13339A>T), GTA475GTT (13344A>T), TGG476TTG (13346G>T), CAG477CAA (13350G>A), TAT479TAC (13356T>C), ACA480ACG (13359A>G), ACT481ACC (13362T>C), GCC482ACA (13363G>A 13365C>A), ACA484ACC (13371A>C), GGT485AGC (13372G>A 13374T>C), ACA486GCA (13375A>G), ACC487GCT (13378A>G 13380C>T), CTT488ACA (13381C>A 13382T>C 13383T>A), GGC490GAC (13388G>A), CGC491CGT (13392C>T), ACC492TTA (13393A>T 13394C>T 13395C>A), TTT493TAT (13397T>A), CGC495CGA (13404C>A), GTC496GTG (13407C>G), ACC498CTT (13411A>C 13412C>T 13413C>T), GGC499GGT (13416C>T), AGC500GGC (13417A>G), TCA501_TGG502insTTTAACGCG (13422_13423insTTTAACGCG), AGC503GGG (13426A>G 13428C>G), GAA504GAG (13431A>G), CGG506CGA (13437G>A), GAA507CAG (13438G>C 13440A>G), GTC508ATT (13441G>A 13443C>T), TAC509TAT (13446C>T), ACC510AAC (13448C>A), TCG511TCA (13452G>A), TAT512AAC (13453T>A 13455T>C), TCA513TCT (13458A>T), CTT514TTG (13459C>T 13461T>G), CCC515CCG (13464C>G), CTG516TTA (13465C>T 13467G>A), AAT517GAG (13468A>G 13470T>G), CTG518TTG (13471C>T), GGC521GGT (13482C>T), GGT522GGC (13485T>C), GCT523GCA (13488T>A), AAG526AAA (13497G>A), ACC534ACA (13521C>A), AGG546CGG (13555A>C), AAT549AAC (13566T>C), GCA570TCA (13627G>T), GTT571GTC (13632T>C), TCC577TCT (13650C>T), ACG579TCG (13654A>T), GGG580GGA (13659G>A), GCG593GCC (13698G>C), CGA596CGC (13707A>C), ACA612ACG (13755A>G), GCA613ACA (13756G>A), CGA615CGT (13764A>T), GTA616GTG (13767A>G), ATC618ATT (13773C>T), ATT619ATC (13776T>C)
139100%27294.8%39 (100%)36 (92.3%)0/0/0/00
Protein mutations:A3T (16329C>T), E12K (16302C>T), *39C (16219T>A)
Codon mutations:GCA3ACA (16329C>T), GAG12AAG (16302C>T), TGA39TGT (16219T>A)
1399.8%25698.5%39 (100%)38 (97.4%)0/0/0/00
1181788.0%38788.4%61 (100%)59 (96.7%)0/0/1/00
Proteins
13222828.1%69198.4%97 (100%)95 (97.9%)0/0/0/00
Protein mutations:A154P (784G>A 786C>G)
Codon mutations:GCC154CCT (784G>A 786C>G), GGC158GGT (772G>A), AAT201AAC (643A>G), GCA203GCG (637T>C), GAT217GAC (595A>G)
46262417.7%99585.9%163 (98.2%)139 (83.7%)3/0/0/00
Protein mutations:L467I (13318T>A 13320G>C), F470K (13327T>A 13328T>A 13329T>G), N474Y (13339A>T), W476L (13346G>T), A482T (13363G>A 13365C>A), G485S (13372G>A 13374T>C), T486A (13375A>G), T487A (13378A>G 13380C>T), L488T (13381C>A 13382T>C 13383T>A), G490D (13388G>A), T492L (13393A>T 13394C>T 13395C>A), F493Y (13397T>A), T498L (13411A>C 13412C>T 13413C>T), S500G (13417A>G), S501_W502insFNA (13422_13423insTTTAACGCG), S503G (13426A>G 13428C>G), E507Q (13438G>C 13440A>G), V508I (13441G>A 13443C>T), T510N (13448C>A), Y512N (13453T>A 13455T>C), N517E (13468A>G 13470T>G), A570S (13627G>T), T579S (13654A>T), A613T (13756G>A)
Codon mutations:GTG466GTC (13317G>C), TTG467ATC (13318T>A 13320G>C), CGG469CGC (13326G>C), TTT470AAG (13327T>A 13328T>A 13329T>G), GTT473GTC (13338T>C), AAT474TAT (13339A>T), GTA475GTT (13344A>T), TGG476TTG (13346G>T), CAG477CAA (13350G>A), TAT479TAC (13356T>C), ACA480ACG (13359A>G), ACT481ACC (13362T>C), GCC482ACA (13363G>A 13365C>A), ACA484ACC (13371A>C), GGT485AGC (13372G>A 13374T>C), ACA486GCA (13375A>G), ACC487GCT (13378A>G 13380C>T), CTT488ACA (13381C>A 13382T>C 13383T>A), GGC490GAC (13388G>A), CGC491CGT (13392C>T), ACC492TTA (13393A>T 13394C>T 13395C>A), TTT493TAT (13397T>A), CGC495CGA (13404C>A), GTC496GTG (13407C>G), ACC498CTT (13411A>C 13412C>T 13413C>T), GGC499GGT (13416C>T), AGC500GGC (13417A>G), TCA501_TGG502insTTTAACGCG (13422_13423insTTTAACGCG), AGC503GGG (13426A>G 13428C>G), GAA504GAG (13431A>G), CGG506CGA (13437G>A), GAA507CAG (13438G>C 13440A>G), GTC508ATT (13441G>A 13443C>T), TAC509TAT (13446C>T), ACC510AAC (13448C>A), TCG511TCA (13452G>A), TAT512AAC (13453T>A 13455T>C), TCA513TCT (13458A>T), CTT514TTG (13459C>T 13461T>G), CCC515CCG (13464C>G), CTG516TTA (13465C>T 13467G>A), AAT517GAG (13468A>G 13470T>G), CTG518TTG (13471C>T), GGC521GGT (13482C>T), GGT522GGC (13485T>C), GCT523GCA (13488T>A), AAG526AAA (13497G>A), ACC534ACA (13521C>A), AGG546CGG (13555A>C), AAT549AAC (13566T>C), GCA570TCA (13627G>T), GTT571GTC (13632T>C), TCC577TCT (13650C>T), ACG579TCG (13654A>T), GGG580GGA (13659G>A), GCG593GCC (13698G>C), CGA596CGC (13707A>C), ACA612ACG (13755A>G), GCA613ACA (13756G>A), CGA615CGT (13764A>T), GTA616GTG (13767A>G), ATC618ATT (13773C>T), ATT619ATC (13776T>C)
139100%27294.8%39 (100%)36 (92.3%)0/0/0/00
Protein mutations:A3T (16329C>T), E12K (16302C>T), *39C (16219T>A)
Codon mutations:GCA3ACA (16329C>T), GAG12AAG (16302C>T), TGA39TGT (16219T>A)
1399.8%25698.5%39 (100%)38 (97.4%)0/0/0/00
Protein mutations:none
Codon mutations:none
1181788.0%38788.4%61 (100%)59 (96.7%)0/0/1/00
Protein mutations:none
Codon mutations:CCG128CMG (27119C>M), GAC132GAT (27132C>T)
*: Inserts / Deletes / Misaligned / Frameshifts

Analysis details

This analysis was performed with panviral2.43