Version 2.16.1
Genome Detective Platform

NGS Details (SRR3458562_Metagenome_example): Peduovirus pv12474III

Assembly

2056 (12 contig(s))
7.0
140
9.03 rpm (after QC)
1
de novo + reference guided assembly
Bcf Tools

Coverage Map

Variant Tables

Assignment

Peduovirus pv12474III (Taxonomy ID: 2844168)
96.6926
96.1765
2
44

Alignment

3842.0 (NT) + 4011.0 (AA) = 7853.0
95.6167
Local, heuristic, nucleotide (BLASTN)

Alignment Detailed Statistics

BeginEndCoverageScoreConcordanceMatchesIdentitiesI/D/M/F*Stop Codons
NT11840329036.1%384293.4%2056 (100%)1988 (96.7%)0/0
Mutations:11840G>T, 11942T>G, 12061C>T, 15961G>A, 16040T>C, 16121C>T, 16186G>C, 16201T>G, 16212G>T, 16213T>G, 16215A>T, 16217C>A, 16218A>C, 17523T>C, 17541T>C, 17589G>A, 17622G>A, 17649G>A, 17738C>G, 17751G>A, 17754G>A, 17786T>C, 17802G>A, 17807C>T, 17808A>G, 17832G>T, 18126G>A, 18240A>G, 18243C>T, 18267G>A, 18586A>T, 18612G>A, 18681T>C, 18690C>A, 18711G>T, 18714A>G, 18747T>C, 21452C>T, 21456G>T, 21494C>T, 21498A>G, 23797T>C, 23799T>K, 23806A>G, 23808C>G, 23872A>G, 25036T>G, 25042T>C, 25050C>A, 25051T>C, 25057T>C, 25063G>C, 25066A>C, 25070G>A, 25078C>T, 25090G>T, 25110G>T, 25111A>T, 25118C>T, 25120C>T, 25126G>C, 25138T>A, 25141G>A, 25159C>A, 32871C>A, 32873T>C, 32877G>A, 32897T>C
CDS
16868.0%42299.1%68 (100%)68 (100%)0/0/0/00
677512.0%48100%9 (100%)9 (100%)0/0/0/01
9918923.5%54292.0%91 (100%)86 (94.5%)0/0/0/00
Protein mutations:V100G (16217C>A 16218A>C), V101D (16215A>T), T102H (16212G>T 16213T>G), R111G (16186G>C)
Codon mutations:GTG100GGT (16217C>A 16218A>C), GTC101GAC (16215A>T), ACC102CAC (16212G>T 16213T>G), AGG106CGG (16201T>G), CGC111GGC (16186G>C), GAG132GAA (16121C>T), TCA159TCG (16040T>C), CTG186TTG (15961G>A)
23365334.4%169197.0%281 (100%)274 (97.5%)0/0/0/00
Protein mutations:M291K (18586A>T), V551I (17807C>T), I558V (17786T>C), G574R (17738C>G)
Codon mutations:GCA237GCG (18747T>C), AAT248AAC (18714A>G), ACC249ACA (18711G>T), GCG256GCT (18690C>A), GCA259GCG (18681T>C), AAC282AAT (18612G>A), ATG291AAG (18586A>T), GGC397GGT (18267G>A), GGG405GGA (18243C>T), AAT406AAC (18240A>G), AGC444AGT (18126G>A), GTC542GTA (17832G>T), GGT550GGC (17808A>G), GTC551ATC (17807C>T), ATC552ATT (17802G>A), ATA558GTA (17786T>C), GCC568GCT (17754G>A), ATC569ATT (17751G>A), GGC574CGC (17738C>G), GCC603GCT (17649G>A), ATC612ATT (17622G>A), TTC623TTT (17589G>A), GAA639GAG (17541T>C), AAA645AAG (17523T>C)
16873.9%40299.0%68 (100%)67 (98.5%)0/0/0/00
52797.1%13484.3%28 (100%)25 (89.3%)0/0/0/00
Protein mutations:V53A (21498A>G), A67E (21456G>T)
Codon mutations:GTG53GCG (21498A>G), CAG54CAA (21494C>T), GCG67GAG (21456G>T), GCG68GCA (21452C>T)
1137.4%7684.4%13 (100%)11 (84.6%)0/0/0/00
Protein mutations:G10R (23806A>G 23808C>G)
Codon mutations:GGT10CGC (23806A>G 23808C>G), AAA13M.G (23797T>C 23799T>K)
2016439.3%33490.5%60 (100%)55 (91.7%)0/0/0/01
Protein mutations:R217Q (25118C>T), L220I (25110G>T), T233M (25070G>A), A240S (25050C>A)
Codon mutations:CTG203CTT (25159C>A), ACC209ACT (25141G>A), GCA210GCT (25138T>A), ACC214ACG (25126G>C), GCG216GCA (25120C>T), CGA217CAA (25118C>T), GGT219GGA (25111A>T), CTT220ATT (25110G>T), GCC226GCA (25090G>T), ACG230ACA (25078C>T), ACG233ATG (25070G>A), CTT234CTG (25066A>C), GCC235GCG (25063G>C), ACA237ACG (25057T>C), AAA239AAG (25051T>C), GCG240TCG (25050C>A), AAA242AAG (25042T>C), GCA244GCC (25036T>G), ATT632ATC (23872A>G)
325415.5%13197.0%23 (100%)22 (95.7%)0/0/0/00
14818615.7%23196.7%39 (100%)37 (94.9%)0/0/0/00
Protein mutations:T150A (32897T>C), T158A (32871C>A 32873T>C)
Codon mutations:ACG150GCG (32897T>C), ACC156ACT (32877G>A), ACG158GCT (32871C>A 32873T>C)
Proteins
16868.0%42299.1%68 (100%)68 (100%)0/0/0/00
Protein mutations:none
Codon mutations:ATA38ATC (11942T>G), TCC72..A (11840G>T)
677512.0%48100%9 (100%)9 (100%)0/0/0/01
Protein mutations:none
Codon mutations:GTG73GTA (12061C>T)
9918923.5%54292.0%91 (100%)86 (94.5%)0/0/0/00
Protein mutations:V100G (16217C>A 16218A>C), V101D (16215A>T), T102H (16212G>T 16213T>G), R111G (16186G>C)
Codon mutations:GTG100GGT (16217C>A 16218A>C), GTC101GAC (16215A>T), ACC102CAC (16212G>T 16213T>G), AGG106CGG (16201T>G), CGC111GGC (16186G>C), GAG132GAA (16121C>T), TCA159TCG (16040T>C), CTG186TTG (15961G>A)
23365334.4%169197.0%281 (100%)274 (97.5%)0/0/0/00
Protein mutations:M291K (18586A>T), V551I (17807C>T), I558V (17786T>C), G574R (17738C>G)
Codon mutations:GCA237GCG (18747T>C), AAT248AAC (18714A>G), ACC249ACA (18711G>T), GCG256GCT (18690C>A), GCA259GCG (18681T>C), AAC282AAT (18612G>A), ATG291AAG (18586A>T), GGC397GGT (18267G>A), GGG405GGA (18243C>T), AAT406AAC (18240A>G), AGC444AGT (18126G>A), GTC542GTA (17832G>T), GGT550GGC (17808A>G), GTC551ATC (17807C>T), ATC552ATT (17802G>A), ATA558GTA (17786T>C), GCC568GCT (17754G>A), ATC569ATT (17751G>A), GGC574CGC (17738C>G), GCC603GCT (17649G>A), ATC612ATT (17622G>A), TTC623TTT (17589G>A), GAA639GAG (17541T>C), AAA645AAG (17523T>C)
16873.9%40299.0%68 (100%)67 (98.5%)0/0/0/00
Protein mutations:none
Codon mutations:none
52797.1%13484.3%28 (100%)25 (89.3%)0/0/0/00
Protein mutations:V53A (21498A>G), A67E (21456G>T)
Codon mutations:GTG53GCG (21498A>G), CAG54CAA (21494C>T), GCG67GAG (21456G>T), GCG68GCA (21452C>T)
1137.4%7684.4%13 (100%)11 (84.6%)0/0/0/00
Protein mutations:G10R (23806A>G 23808C>G)
Codon mutations:GGT10CGC (23806A>G 23808C>G), AAA13M.G (23797T>C 23799T>K)
2016439.3%33490.5%60 (100%)55 (91.7%)0/0/0/01
Protein mutations:R217Q (25118C>T), L220I (25110G>T), T233M (25070G>A), A240S (25050C>A)
Codon mutations:CTG203CTT (25159C>A), ACC209ACT (25141G>A), GCA210GCT (25138T>A), ACC214ACG (25126G>C), GCG216GCA (25120C>T), CGA217CAA (25118C>T), GGT219GGA (25111A>T), CTT220ATT (25110G>T), GCC226GCA (25090G>T), ACG230ACA (25078C>T), ACG233ATG (25070G>A), CTT234CTG (25066A>C), GCC235GCG (25063G>C), ACA237ACG (25057T>C), AAA239AAG (25051T>C), GCG240TCG (25050C>A), AAA242AAG (25042T>C), GCA244GCC (25036T>G), ATT632ATC (23872A>G)
325415.5%13197.0%23 (100%)22 (95.7%)0/0/0/00
Protein mutations:none
Codon mutations:none
14818615.7%23196.7%39 (100%)37 (94.9%)0/0/0/00
Protein mutations:T150A (32897T>C), T158A (32871C>A 32873T>C)
Codon mutations:ACG150GCG (32897T>C), ACC156ACT (32877G>A), ACG158GCT (32871C>A 32873T>C)
*: Inserts / Deletes / Misaligned / Frameshifts

Analysis details

This analysis was performed with panviral2.43