NGS Details (SRR3458562_Metagenome_example): Lambdavirus lambda

Assembly

980 (5 contig(s))
8.4
80
5.16 rpm (after QC)
1
de novo + reference guided assembly
Bcf Tools

Coverage Map

Variant Tables

Assignment

Lambdavirus lambda (Taxonomy ID: 10710)
96.319
97.0149
4
73

Alignment

1794.0 (NT) + 2555.0 (AA) = 4349.0
94.5435
Local, heuristic, nucleotide (BLASTN)

Alignment Detailed Statistics

BeginEndCoverageScoreConcordanceMatchesIdentitiesI/D/M/F*Stop Codons
NT21257456802.0%179492.7%978 (99.8%)942 (96.1%)0/2
Mutations:21273T>G, 22473G>T, 22478A>T, 22500T>A, 22511C>A, 22518A>C, 22538C>T, 22539T>G, 22540G>A, 22541T>A, 22548T>C, 22550T>G, 22551G>A, 22560G>A, 22563T>G, 22564T>G, 22566T>A, 22567G>T, 22568delA, 22579A>C, 22581delA, 22585T>C, 22588A>G, 22589T>C, 22591A>C, 22594T>G, 22600T>G, 34319C>T, 34344A>M, 34384T>C, 38393C>A, 45215G>A, 45419T>C, 45498A>G, 45546G>A, 45567T>G, 45645A>G, 45680G>C
CDS
77997.3%16193.6%23 (100%)21 (91.3%)0/0/0/00
Protein mutations:I82S (21273T>G)
Codon mutations:ATC82AGC (21273T>G)
16719514.9%15673.9%29 (100%)23 (79.3%)0/0/0/02
Protein mutations:W167C (22473G>T), K169M (22478A>T), S180* (22511C>A), A189V (22538C>T 22539T>G), V190N (22540G>A 22541T>A), M193R (22550T>G 22551G>A)
Codon mutations:TGG167TGT (22473G>T), AAG169ATG (22478A>T), CGT176CGA (22500T>A), TCA180TAA (22511C>A), GCA182GCC (22518A>C), GCT189GTG (22538C>T 22539T>G), GTC190AAC (22540G>A 22541T>A), GTT192GTC (22548T>C), ATG193AGA (22550T>G 22551G>A)
11965.5%11378.5%19 (100%)18 (94.7%)0/0/1/00
15657.1%34698.9%56 (100%)55 (98.2%)0/0/0/00
1108100%68198.6%108 (100%)107 (99.1%)0/0/0/01
Protein mutations:R105G (45498A>G)
Codon mutations:TTG10TTA (45215G>A), TTT78TTC (45419T>C), AGA105GGA (45498A>G)
1106100%67198.5%106 (100%)105 (99.1%)0/0/0/01
Protein mutations:R103G (45498A>G)
Codon mutations:TTG8TTA (45215G>A), TTT76TTC (45419T>C), AGA103GGA (45498A>G)
16138.4%42798.2%61 (100%)61 (100%)0/0/0/00
Proteins
77997.3%16193.6%23 (100%)21 (91.3%)0/0/0/00
Protein mutations:I82S (21273T>G)
Codon mutations:ATC82AGC (21273T>G)
16719514.9%15673.9%29 (100%)23 (79.3%)0/0/0/02
Protein mutations:W167C (22473G>T), K169M (22478A>T), S180* (22511C>A), A189V (22538C>T 22539T>G), V190N (22540G>A 22541T>A), M193R (22550T>G 22551G>A)
Codon mutations:TGG167TGT (22473G>T), AAG169ATG (22478A>T), CGT176CGA (22500T>A), TCA180TAA (22511C>A), GCA182GCC (22518A>C), GCT189GTG (22538C>T 22539T>G), GTC190AAC (22540G>A 22541T>A), GTT192GTC (22548T>C), ATG193AGA (22550T>G 22551G>A)
11965.5%11378.5%19 (100%)18 (94.7%)0/0/1/00
Protein mutations:none
Codon mutations:TTT5TKT (34344A>M), CAG13CAA (34319C>T)
15657.1%34698.9%56 (100%)55 (98.2%)0/0/0/00
Protein mutations:none
Codon mutations:CGA12AGA (38393C>A)
1108100%68198.6%108 (100%)107 (99.1%)0/0/0/01
Protein mutations:R105G (45498A>G)
Codon mutations:TTG10TTA (45215G>A), TTT78TTC (45419T>C), AGA105GGA (45498A>G)
1106100%67198.5%106 (100%)105 (99.1%)0/0/0/01
Protein mutations:R103G (45498A>G)
Codon mutations:TTG8TTA (45215G>A), TTT76TTC (45419T>C), AGA103GGA (45498A>G)
16138.4%42798.2%61 (100%)61 (100%)0/0/0/00
Protein mutations:none
Codon mutations:GTA2GTG (45498A>G), TCG18TCA (45546G>A), CGT25CGG (45567T>G), AAA51AAG (45645A>G), GGC63.C. (45680G>C)
*: Inserts / Deletes / Misaligned / Frameshifts

Analysis details

This analysis was performed with panviral2.43