Version 2.16.1
Genome Detective Platform

NGS Details (SRR3458562_Metagenome_example): Enterobacteria phage mEp460

Assembly

624 (4 contig(s))
8.2
57
3.68 rpm (after QC)
0
de novo + reference guided assembly
Bcf Tools

Coverage Map

Variant Tables

Assignment

Enterobacteria phage mEp460 (Taxonomy ID: 1147152)
89.7436
91.2791
2
59

Alignment

992.0 (NT) + 1025.0 (AA) = 2017.0
85.7204
Local, heuristic, nucleotide (BLASTN)

Alignment Detailed Statistics

BeginEndCoverageScoreConcordanceMatchesIdentitiesI/D/M/F*Stop Codons
NT19201428721.4%99279.5%624 (100%)560 (89.7%)0/0
Mutations:19211C>T, 19232G>A, 19247C>T, 19254T>C, 19256C>T, 19271T>C, 19273T>C, 19274T>C, 19278T>C, 19283G>T, 19289C>T, 19292C>T, 19298G>T, 19316C>T, 19322G>C, 19330A>G, 19335C>A, 19340C>A, 19344T>A, 19384C>T, 19388T>A, 19412A>G, 19414T>A, 19431G>T, 19433G>A, 19435A>G, 19442G>C, 19448A>G, 19451G>T, 19460C>T, 19466T>A, 19472A>G, 19475G>A, 39699A>T, 39702G>A, 39704T>C, 39723A>G, 39730A>G, 39733G>A, 39748G>A, 39749A>G, 39750C>T, 39758T>A, 39794C>T, 39817A>G, 42294A>T, 42300T>G, 42309G>A, 42312T>C, 42314A>G, 42318A>G, 42322A>C, 42323A>G, 42324G>T, 42333T>G, 42785G>A, 42788T>A, 42797A>G, 42800C>A, 42810T>C, 42815T>G, 42863T>C, 42865T>A, 42866A>C
CDS
401327.7%54789.7%93 (100%)84 (90.3%)0/0/0/00
Protein mutations:Y57H (19254T>C 19256C>T), I63T (19273T>C 19274T>C), D82G (19330A>G), S87T (19344T>A), A100V (19384C>T), L110Q (19414T>A), A116S (19431G>T 19433G>A), H117R (19435A>G)
Codon mutations:TCC42TCT (19211C>T), GGG49GGA (19232G>A), GAC54GAT (19247C>T), TAC57CAT (19254T>C 19256C>T), GTT62GTC (19271T>C), ATT63ACC (19273T>C 19274T>C), TTG65CTG (19278T>C), GTG66GTT (19283G>T), GGC68GGT (19289C>T), TTC69TTT (19292C>T), CCG71CCT (19298G>T), ATC77ATT (19316C>T), GTG79GTC (19322G>C), GAT82GGT (19330A>G), CGA84AGA (19335C>A), CCC85CCA (19340C>A), TCG87ACG (19344T>A), GCC100GTC (19384C>T), CGT101CGA (19388T>A), GAA109GAG (19412A>G), CTG110CAG (19414T>A), GCG116TCA (19431G>T 19433G>A), CAT117CGT (19435A>G), GCG119GCC (19442G>C), GCA121GCG (19448A>G), GTG122GTT (19451G>T), AAC125AAT (19460C>T), GCT127GCA (19466T>A), GCA129GCG (19472A>G), AAG130AAA (19475G>A)
2392515.2%6684.6%13 (100%)12 (92.3%)0/0/0/01
Protein mutations:I243T (39704T>C)
Codon mutations:GCA241GCT (39699A>T), GAG242GAA (39702G>A), ATA243ACA (39704T>C), ACA249ACG (39723A>G)
759320.4%10691.4%19 (100%)17 (89.5%)0/0/0/01
Protein mutations:K82R (42314A>G), K85R (42322A>C 42323A>G 42324G>T)
Codon mutations:ACA75ACT (42294A>T), CTT77CTG (42300T>G), AAG80AAA (42309G>A), ATT81ATC (42312T>C), AAA82AGA (42314A>G), GAA83GAG (42318A>G), AAG85CGT (42322A>C 42323A>G 42324G>T), GCT88GCG (42333T>G)
11514.3%9089.1%15 (100%)14 (93.3%)0/0/0/00
195018.0%21692.3%32 (100%)30 (93.8%)0/0/0/00
Protein mutations:F30L (42810T>C), I48N (42865T>A 42866A>C)
Codon mutations:GCG21GCA (42785G>A), TCT22TCA (42788T>A), CAA25CAG (42797A>G), ATC26ATA (42800C>A), TTT30CTT (42810T>C), CTT31CTG (42815T>G), GGT47GGC (42863T>C), ATA48AAC (42865T>A 42866A>C)
Proteins
401327.7%54789.7%93 (100%)84 (90.3%)0/0/0/00
Protein mutations:Y57H (19254T>C 19256C>T), I63T (19273T>C 19274T>C), D82G (19330A>G), S87T (19344T>A), A100V (19384C>T), L110Q (19414T>A), A116S (19431G>T 19433G>A), H117R (19435A>G)
Codon mutations:TCC42TCT (19211C>T), GGG49GGA (19232G>A), GAC54GAT (19247C>T), TAC57CAT (19254T>C 19256C>T), GTT62GTC (19271T>C), ATT63ACC (19273T>C 19274T>C), TTG65CTG (19278T>C), GTG66GTT (19283G>T), GGC68GGT (19289C>T), TTC69TTT (19292C>T), CCG71CCT (19298G>T), ATC77ATT (19316C>T), GTG79GTC (19322G>C), GAT82GGT (19330A>G), CGA84AGA (19335C>A), CCC85CCA (19340C>A), TCG87ACG (19344T>A), GCC100GTC (19384C>T), CGT101CGA (19388T>A), GAA109GAG (19412A>G), CTG110CAG (19414T>A), GCG116TCA (19431G>T 19433G>A), CAT117CGT (19435A>G), GCG119GCC (19442G>C), GCA121GCG (19448A>G), GTG122GTT (19451G>T), AAC125AAT (19460C>T), GCT127GCA (19466T>A), GCA129GCG (19472A>G), AAG130AAA (19475G>A)
2392515.2%6684.6%13 (100%)12 (92.3%)0/0/0/01
Protein mutations:I243T (39704T>C)
Codon mutations:GCA241GCT (39699A>T), GAG242GAA (39702G>A), ATA243ACA (39704T>C), ACA249ACG (39723A>G)
759320.4%10691.4%19 (100%)17 (89.5%)0/0/0/01
Protein mutations:K82R (42314A>G), K85R (42322A>C 42323A>G 42324G>T)
Codon mutations:ACA75ACT (42294A>T), CTT77CTG (42300T>G), AAG80AAA (42309G>A), ATT81ATC (42312T>C), AAA82AGA (42314A>G), GAA83GAG (42318A>G), AAG85CGT (42322A>C 42323A>G 42324G>T), GCT88GCG (42333T>G)
11514.3%9089.1%15 (100%)14 (93.3%)0/0/0/00
Protein mutations:none
Codon mutations:none
195018.0%21692.3%32 (100%)30 (93.8%)0/0/0/00
Protein mutations:F30L (42810T>C), I48N (42865T>A 42866A>C)
Codon mutations:GCG21GCA (42785G>A), TCT22TCA (42788T>A), CAA25CAG (42797A>G), ATC26ATA (42800C>A), TTT30CTT (42810T>C), CTT31CTG (42815T>G), GGT47GGC (42863T>C), ATA48AAC (42865T>A 42866A>C)
*: Inserts / Deletes / Misaligned / Frameshifts

Analysis details

This analysis was performed with panviral2.43