Version 2.16.3
Genome Detective Platform

NGS Details (SRR3458562_Metagenome_example): Wadgaonvirus wv5004651

Assembly

3584 (11 contig(s))
18.4
655
42.26 rpm (after QC)
0
de novo + reference guided assembly
Bcf Tools

Coverage Map

Variant Tables

Assignment

Wadgaonvirus wv5004651 (Taxonomy ID: 2956672)
96.8489
94.9506
6
57

Alignment

6686.0 (NT) + 5310.0 (AA) = 11996.0
94.0255
Local, heuristic, nucleotide (BLASTN)

Alignment Detailed Statistics

BeginEndCoverageScoreConcordanceMatchesIdentitiesI/D/M/F*Stop Codons
NT20106382219.1%668693.4%3583 (99.9%)3473 (96.8%)3/1
Mutations:20108A>T, 20110A>C, 20117G>A, 20120T>C, 20123T>C, 20147G>A, 20153C>A, 20155G>A, 20159C>G, 20181A>G, 20182G>A, 20186A>G, 20191A>T, 20207G>A, 20236A>T, 20254G>T, 20259C>G, 20272T>C, 20281C>T, 20287T>C, 20295G>A, 20305T>G, 20314T>A, 20317C>T, 20328T>C, 20329T>G, 20383T>C, 20406G>A, 20416A>G, 20425G>A, 20429G>C, 20440T>A, 30434A>C, 30459G>A, 30460C>G, 30467C>T, 30472T>C, 30473C>A, 30476T>A, 30651G>A, 30655T>G, 30662T>G, 30666T>C, 30723T>C, 30900G>A, 30945A>G, 30957G>A, 31264C>T, 31297T>C, 31305G>A, 31516T>C, 31521A>G, 31528G>T, 31534G>A, 31540C>T, 31552T>C, 31555T>C, 31558A>T, 31574C>T, 31585G>C, 31591T>C, 31597C>T, 31654C>T, 31672G>A, 31673T>G, 31684C>G, 31696G>T, 31705G>A, 31714T>C, 31788C>T, 31832C>T, 31904A>C, 31912G>T, 32567T>C, 32597A>G, 33241A>T, 33250A>G, 33329T>C, 33371C>T, 33380G>A, 33407T>C, 33488C>T, 33500G>A, 33518G>A, 33756A>G, 33758T>C, 33759A>T, 33761A>T, 33763A>T, 33794C>T, 34981T>A, 34996T>C, 34997A>T, 35172_35173insA, 35202A>G, 35254_35255insC, 35270A>G, 35479delA, 35497T>C, 35617T>G, 35656T>C, 35671C>T, 35719T>C, 35740T>C, 35746G>C, 35746_35747insC, 35748G>A, 35757G>A, 35772T>C, 37931A>G, 37944A>G, 37952C>A, 38054G>A, 38219G>C
CDS
4875033.4%10289.5%17 (100%)15 (88.2%)0/0/0/01
Protein mutations:E487D (20108A>T), N488T (20110A>C)
Codon mutations:GAA487GAT (20108A>T), AAC488ACC (20110A>C), CCG490CCA (20117G>A), CGT491CGC (20120T>C), AAT492AAC (20123T>C), AGG500AGA (20147G>A), GCC502GCA (20153C>A), TGA503TAA (20155G>A)
19748.3%60790.3%97 (100%)88 (90.7%)0/0/0/00
Protein mutations:H2D (20159C>G), E9G (20181A>G 20182G>A), I11V (20186A>G), V18I (20207G>A), T35S (20259C>G), C47Y (20295G>A), V58A (20328T>C 20329T>G), S84N (20406G>A), E92Q (20429G>C)
Codon mutations:CAT2GAT (20159C>G), GAG9GGA (20181A>G 20182G>A), ATT11GTT (20186A>G), GCA12GCT (20191A>T), GTT18ATT (20207G>A), ACA27ACT (20236A>T), ACG33ACT (20254G>T), ACT35AGT (20259C>G), GCT39GCC (20272T>C), GTC42GTT (20281C>T), ATT44ATC (20287T>C), TGT47TAT (20295G>A), CTT50CTG (20305T>G), CCT53CCA (20314T>A), GGC54GGT (20317C>T), GTT58GCG (20328T>C 20329T>G), GAT76GAC (20383T>C), AGC84AAC (20406G>A), ACA87ACG (20416A>G), TCG90TCA (20425G>A), GAA92CAA (20429G>C), GCT95GCA (20440T>A)
65691.1%31893.8%51 (100%)48 (94.1%)0/0/0/01
Protein mutations:L11F (30473C>A), R12G (30472T>C), A16L (30459G>A 30460C>G)
Codon mutations:CCA10CCT (30476T>A), TTG11TTT (30473C>A), AGG12GGG (30472T>C), CTG13CTA (30467C>T), GCG16CTG (30459G>A 30460C>G), CCT24CCG (30434A>C)
116167.7%70097.2%109 (100%)106 (97.2%)0/0/0/01
Protein mutations:I154L (30662T>G), K156T (30655T>G)
Codon mutations:TAC55TAT (30957G>A), CGT59CGC (30945A>G), GCC74GCT (30900G>A), AAA133AAG (30723T>C), GCA152GCG (30666T>C), ATC154CTC (30662T>G), AAA156ACA (30655T>G), AAC157AAT (30651G>A)
115960.4%61396.8%96 (100%)92 (95.8%)0/0/0/01
Protein mutations:S18N (31305G>A), N90S (31521A>G), E111D (31585G>C), S141A (31673T>G)
Codon mutations:TTC4TTT (31264C>T), CCT15CCC (31297T>C), AGC18AAC (31305G>A), CTT88CTC (31516T>C), AAT90AGT (31521A>G), GTG92GTT (31528G>T), GGG94GGA (31534G>A), TTC96TTT (31540C>T), ATT100ATC (31552T>C), GGT101GGC (31555T>C), GGA102GGT (31558A>T), CTG108TTG (31574C>T), GAG111GAC (31585G>C), TGT113TGC (31591T>C), ATC115ATT (31597C>T), CGC134CGT (31654C>T), GCG140GCA (31672G>A), TCT141GCT (31673T>G), GTC144GTG (31684C>G), ACG148ACT (31696G>T), GCG151GCA (31705G>A), GTT154GTC (31714T>C)
17173.2%42896.8%71 (100%)69 (97.2%)0/0/0/00
Protein mutations:S65I (31912G>T)
Codon mutations:CTG24TTG (31788C>T), GGC38GGT (31832C>T), CTA62CTC (31904A>C), AGT65ATT (31912G>T)
13328.9%20296.2%33 (100%)32 (97.0%)0/0/0/00
11455429.8%101194.7%165 (100%)158 (95.8%)0/0/0/01
Protein mutations:S154T (33761A>T 33763A>T), L155Q (33758T>C 33759A>T), L156P (33756A>G), Y325H (33250A>G), S328T (33241A>T)
Codon mutations:AAG143AAA (33794C>T), TCT154ACA (33761A>T 33763A>T), CTA155CAG (33758T>C 33759A>T), CTC156CCC (33756A>G), GCC235GCT (33518G>A), AAC241AAT (33500G>A), CAG245CAA (33488C>T), GAA272GAG (33407T>C), AGC281AGT (33380G>A), CTG284CTA (33371C>T), CTA298CTG (33329T>C), TAT325CAT (33250A>G), TCG328ACG (33241A>T), AAT542AAC (32597A>G), GCA552GCG (32567T>C)
16221.9%41496.5%62 (100%)60 (96.8%)0/0/0/00
Protein mutations:L53Q (34996T>C 34997A>T)
Codon mutations:CTA53CAG (34996T>C 34997A>T), GTA58GTT (34981T>A)
160100%25066.7%60 (100%)59 (98.3%)0/0/1/11
111873.4%33853.9%91 (98.9%)86 (93.5%)1/0/2/20
Protein mutations:V91_R92insX (35746_35747insC), R92H (35748G>A), R95Q (35757G>A), L100P (35772T>C)
Codon mutations:AAA2AA- (35479delA), CGT8CGC (35497T>C), CGT48CGG (35617T>G), TCT61TCC (35656T>C), GGC66GGT (35671C>T), GGT82GGC (35719T>C), ACT89ACC (35740T>C), GTG91GTC (35746G>C), GTG91_CGC92insC-- (35746_35747insC), CGC92CAC (35748G>A), CGA95CAA (35757G>A), CTA100CCA (35772T>C)
128.0%642.9%2 (100%)1 (50.0%)0/0/0/00
15420.5%32195.5%54 (100%)51 (94.4%)0/0/0/00
Protein mutations:M33I (37952C>A), I36T (37944A>G)
Codon mutations:ATG33ATT (37952C>A), ATT36ACT (37944A>G), ATT40ATC (37931A>G)
120.6%00%2 (100%)0 (0%)0/0/0/00
Protein mutations:M1I (38219G>C)
Codon mutations:ATG1ATC (38219G>C)
Proteins
4875033.4%10289.5%17 (100%)15 (88.2%)0/0/0/01
Protein mutations:E487D (20108A>T), N488T (20110A>C)
Codon mutations:GAA487GAT (20108A>T), AAC488ACC (20110A>C), CCG490CCA (20117G>A), CGT491CGC (20120T>C), AAT492AAC (20123T>C), AGG500AGA (20147G>A), GCC502GCA (20153C>A), TGA503TAA (20155G>A)
19748.3%60790.3%97 (100%)88 (90.7%)0/0/0/00
Protein mutations:H2D (20159C>G), E9G (20181A>G 20182G>A), I11V (20186A>G), V18I (20207G>A), T35S (20259C>G), C47Y (20295G>A), V58A (20328T>C 20329T>G), S84N (20406G>A), E92Q (20429G>C)
Codon mutations:CAT2GAT (20159C>G), GAG9GGA (20181A>G 20182G>A), ATT11GTT (20186A>G), GCA12GCT (20191A>T), GTT18ATT (20207G>A), ACA27ACT (20236A>T), ACG33ACT (20254G>T), ACT35AGT (20259C>G), GCT39GCC (20272T>C), GTC42GTT (20281C>T), ATT44ATC (20287T>C), TGT47TAT (20295G>A), CTT50CTG (20305T>G), CCT53CCA (20314T>A), GGC54GGT (20317C>T), GTT58GCG (20328T>C 20329T>G), GAT76GAC (20383T>C), AGC84AAC (20406G>A), ACA87ACG (20416A>G), TCG90TCA (20425G>A), GAA92CAA (20429G>C), GCT95GCA (20440T>A)
65691.1%31893.8%51 (100%)48 (94.1%)0/0/0/01
Protein mutations:L11F (30473C>A), R12G (30472T>C), A16L (30459G>A 30460C>G)
Codon mutations:CCA10CCT (30476T>A), TTG11TTT (30473C>A), AGG12GGG (30472T>C), CTG13CTA (30467C>T), GCG16CTG (30459G>A 30460C>G), CCT24CCG (30434A>C)
116167.7%70097.2%109 (100%)106 (97.2%)0/0/0/01
Protein mutations:I154L (30662T>G), K156T (30655T>G)
Codon mutations:TAC55TAT (30957G>A), CGT59CGC (30945A>G), GCC74GCT (30900G>A), AAA133AAG (30723T>C), GCA152GCG (30666T>C), ATC154CTC (30662T>G), AAA156ACA (30655T>G), AAC157AAT (30651G>A)
115960.4%61396.8%96 (100%)92 (95.8%)0/0/0/01
Protein mutations:S18N (31305G>A), N90S (31521A>G), E111D (31585G>C), S141A (31673T>G)
Codon mutations:TTC4TTT (31264C>T), CCT15CCC (31297T>C), AGC18AAC (31305G>A), CTT88CTC (31516T>C), AAT90AGT (31521A>G), GTG92GTT (31528G>T), GGG94GGA (31534G>A), TTC96TTT (31540C>T), ATT100ATC (31552T>C), GGT101GGC (31555T>C), GGA102GGT (31558A>T), CTG108TTG (31574C>T), GAG111GAC (31585G>C), TGT113TGC (31591T>C), ATC115ATT (31597C>T), CGC134CGT (31654C>T), GCG140GCA (31672G>A), TCT141GCT (31673T>G), GTC144GTG (31684C>G), ACG148ACT (31696G>T), GCG151GCA (31705G>A), GTT154GTC (31714T>C)
17173.2%42896.8%71 (100%)69 (97.2%)0/0/0/00
Protein mutations:S65I (31912G>T)
Codon mutations:CTG24TTG (31788C>T), GGC38GGT (31832C>T), CTA62CTC (31904A>C), AGT65ATT (31912G>T)
13328.9%20296.2%33 (100%)32 (97.0%)0/0/0/00
Protein mutations:none
Codon mutations:none
11455429.8%101194.7%165 (100%)158 (95.8%)0/0/0/01
Protein mutations:S154T (33761A>T 33763A>T), L155Q (33758T>C 33759A>T), L156P (33756A>G), Y325H (33250A>G), S328T (33241A>T)
Codon mutations:AAG143AAA (33794C>T), TCT154ACA (33761A>T 33763A>T), CTA155CAG (33758T>C 33759A>T), CTC156CCC (33756A>G), GCC235GCT (33518G>A), AAC241AAT (33500G>A), CAG245CAA (33488C>T), GAA272GAG (33407T>C), AGC281AGT (33380G>A), CTG284CTA (33371C>T), CTA298CTG (33329T>C), TAT325CAT (33250A>G), TCG328ACG (33241A>T), AAT542AAC (32597A>G), GCA552GCG (32567T>C)
16221.9%41496.5%62 (100%)60 (96.8%)0/0/0/00
Protein mutations:L53Q (34996T>C 34997A>T)
Codon mutations:CTA53CAG (34996T>C 34997A>T), GTA58GTT (34981T>A)
160100%25066.7%60 (100%)59 (98.3%)0/0/1/11
Protein mutations:none
Codon mutations:ACG59-CG (35479delA)
111873.4%33853.9%91 (98.9%)86 (93.5%)1/0/2/20
Protein mutations:V91_R92insX (35746_35747insC), R92H (35748G>A), R95Q (35757G>A), L100P (35772T>C)
Codon mutations:AAA2AA- (35479delA), CGT8CGC (35497T>C), CGT48CGG (35617T>G), TCT61TCC (35656T>C), GGC66GGT (35671C>T), GGT82GGC (35719T>C), ACT89ACC (35740T>C), GTG91GTC (35746G>C), GTG91_CGC92insC-- (35746_35747insC), CGC92CAC (35748G>A), CGA95CAA (35757G>A), CTA100CCA (35772T>C)
128.0%642.9%2 (100%)1 (50.0%)0/0/0/00
Protein mutations:none
Codon mutations:none
15420.5%32195.5%54 (100%)51 (94.4%)0/0/0/00
Protein mutations:M33I (37952C>A), I36T (37944A>G)
Codon mutations:ATG33ATT (37952C>A), ATT36ACT (37944A>G), ATT40ATC (37931A>G)
120.6%00%2 (100%)0 (0%)0/0/0/00
Protein mutations:M1I (38219G>C)
Codon mutations:ATG1ATC (38219G>C)
*: Inserts / Deletes / Misaligned / Frameshifts

Analysis details

This analysis was performed with panviral2.43