NGS Details (SRR3458562_Metagenome_example): Wanchaivirus HK106

Assembly

503 (4 contig(s))
6.0
31
2.00 rpm (after QC)
5
de novo + reference guided assembly
Bcf Tools

Coverage Map

Variant Tables

Assignment

Wanchaivirus HK106 (Taxonomy ID: 2169920)
95.6262
95.0
2
65

Alignment

928.0 (NT) + 1166.0 (AA) = 2094.0
93.108
Local, heuristic, nucleotide (BLASTN)

Alignment Detailed Statistics

BeginEndCoverageScoreConcordanceMatchesIdentitiesI/D/M/F*Stop Codons
NT19919273841.2%92892.2%503 (100%)481 (95.6%)0/0
Mutations:19949A>G, 19964A>T, 19976T>K, 19985T>K, 20000C>T, 20003G>R, 20006T>C, 20009G>C, 20012C>T, 20019G>A, 20024A>K, 20028C>G, 20036C>A, 20037G>A, 22788C>G, 22793G>T, 22814T>C, 22848G>A, 27089A>G, 27119G>A, 27258G>K, 27324G>A
CDS
21125112.3%24181.1%41 (100%)36 (87.8%)0/0/1/00
Protein mutations:G244R (20019G>A), L247V (20028C>G), A250T (20037G>A)
Codon mutations:CCA220CCG (19949A>G), CCA225CCT (19964A>T), GGT229GGK (19976T>K), ATT232ATK (19985T>K), GAC237GAT (20000C>T), GGG238GGR (20003G>R), CGT239CGC (20006T>C), GGG240GGC (20009G>C), GTC241GTT (20012C>T), GGG244AGG (20019G>A), CGA245CGK (20024A>K), CTG247GTG (20028C>G), TCC249TCA (20036C>A), GCA250ACA (20037G>A)
275653.6%22391.4%30 (100%)29 (96.7%)0/0/0/01
Protein mutations:T54A (22814T>C)
Codon mutations:ACC42ACT (22848G>A), ACC54GCC (22814T>C)
115522.3%29696.4%45 (100%)44 (97.8%)0/0/0/00
95378.9%28785.9%45 (100%)43 (95.6%)0/0/1/00
355335.8%119100%19 (100%)19 (100%)0/0/0/01
Proteins
21125112.3%24181.1%41 (100%)36 (87.8%)0/0/1/00
Protein mutations:G244R (20019G>A), L247V (20028C>G), A250T (20037G>A)
Codon mutations:CCA220CCG (19949A>G), CCA225CCT (19964A>T), GGT229GGK (19976T>K), ATT232ATK (19985T>K), GAC237GAT (20000C>T), GGG238GGR (20003G>R), CGT239CGC (20006T>C), GGG240GGC (20009G>C), GTC241GTT (20012C>T), GGG244AGG (20019G>A), CGA245CGK (20024A>K), CTG247GTG (20028C>G), TCC249TCA (20036C>A), GCA250ACA (20037G>A)
275653.6%22391.4%30 (100%)29 (96.7%)0/0/0/01
Protein mutations:T54A (22814T>C)
Codon mutations:ACC42ACT (22848G>A), ACC54GCC (22814T>C)
115522.3%29696.4%45 (100%)44 (97.8%)0/0/0/00
Protein mutations:none
Codon mutations:GAC36GAT (27119G>A), AAT46AAC (27089A>G)
95378.9%28785.9%45 (100%)43 (95.6%)0/0/1/00
Protein mutations:none
Codon mutations:GGC28GGT (27324G>A), TTC50TTM (27258G>K)
355335.8%119100%19 (100%)19 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
*: Inserts / Deletes / Misaligned / Frameshifts

Analysis details

This analysis was performed with panviral2.43