NGS Details (SRR3458562_Metagenome_example): Friend murine leukemia virus

Assembly

280 (2 contig(s))
2.1
5
0.32 rpm (after QC)
0
de novo + reference guided assembly
Bcf Tools

Coverage Map

Variant Tables

Assignment

Friend murine leukemia virus (Taxonomy ID: 11795)
75.0903
79.5699
0
3

Alignment

266.0 (NT) + 511.0 (AA) = 777.0
67.7419
Global, seeded, nucleotide + amino acids (AGA)

Alignment Detailed Statistics

BeginEndCoverageScoreConcordanceMatchesIdentitiesI/D/M/F*Stop Codons
NT523375833.4%26649.1%277 (98.9%)208 (74.3%)0/3
Mutations:5235T>C, 5244A>G, 5259T>A, 5268A>G, 5271A>G, 5278A>C, 5283A>C, 5284A>T, 5286C>A, 5289G>A, 5295T>C, 5310G>T, 5311C>A, 5313T>G, 5315C>A, 5316A>G, 5323T>G, 5326_5328delAGG, 5329G>A, 5331C>T, 5338C>G, 5343G>T, 5346T>C, 5350C>T, 5351T>A, 5352A>C, 5355C>T, 5360A>T, 5361T>C, 5362C>G, 5364A>G, 5370C>T, 5376G>C, 7454A>G, 7457G>A, 7460A>C, 7463A>G, 7466G>C, 7472T>A, 7478A>C, 7485G>C, 7493T>C, 7496C>T, 7499T>C, 7502T>A, 7509A>T, 7514C>A, 7515C>G, 7517A>G, 7520A>T, 7522G>A, 7527A>T, 7528G>C, 7535C>A, 7539T>C, 7547G>A, 7551C>T, 7553C>A, 7554T>G, 7555C>A, 7556T>C, 7557C>A, 7559G>A, 7560A>C, 7563C>A, 7569C>G, 7570T>A, 7572T>A, 7573T>G, 7574T>A
CDS
100510534.1%25883.0%48 (98.0%)38 (77.6%)0/1/0/00
Protein mutations:I1022L (5284A>T 5286C>A), L1031M (5311C>A 5313T>G), A1032E (5315C>A 5316A>G), S1035A (5323T>G), R1036del (5326_5328delAGG), D1037N (5329G>A 5331C>T), L1040V (5338C>G), L1044Y (5350C>T 5351T>A 5352A>C), Y1047F (5360A>T 5361T>C), R1048G (5362C>G 5364A>G)
Codon mutations:TGT1005TGC (5235T>C), AGA1008AGG (5244A>G), GGT1013GGA (5259T>A), GAA1016GAG (5268A>G), AGA1017AGG (5271A>G), AGG1020CGG (5278A>C), ACA1021ACC (5283A>C), ATC1022TTA (5284A>T 5286C>A), AAG1023AAA (5289G>A), ACT1025ACC (5295T>C), ACG1030ACT (5310G>T), CTT1031ATG (5311C>A 5313T>G), GCA1032GAG (5315C>A 5316A>G), TCT1035GCT (5323T>G), AGG1036del (5326_5328delAGG), GAC1037AAT (5329G>A 5331C>T), CTC1040GTC (5338C>G), CTG1041CTT (5343G>T), CTT1042CTC (5346T>C), CTA1044TAC (5350C>T 5351T>A 5352A>C), GCC1045GCT (5355C>T), TAT1047TTC (5360A>T 5361T>C), CGA1048GGG (5362C>G 5364A>G), CGC1050CGT (5370C>T), ACG1052ACC (5376G>C)
5596036.6%25382.7%45 (100%)36 (80.0%)0/0/0/00
Protein mutations:E571Q (7485G>C), T579S (7509A>T), L581V (7515C>G 7517A>G), R583K (7522G>A), S594D (7554T>G 7555C>A 7556T>C), Q595K (7557C>A 7559G>A), Q597K (7563C>A), L599E (7569C>G 7570T>A), F600R (7572T>A 7573T>G 7574T>A)
Codon mutations:AAA560AAG (7454A>G), GAG561GAA (7457G>A), GGA562GGC (7460A>C), GGA563GGG (7463A>G), CTG564CTC (7466G>C), GCT566GCA (7472T>A), CTA568CTC (7478A>C), GAA571CAA (7485G>C), TGT573TGC (7493T>C), TTC574TTT (7496C>T), TAT575TAC (7499T>C), GCT576GCA (7502T>A), ACA579TCA (7509A>T), GGC580GGA (7514C>A), CTA581GTG (7515C>G 7517A>G), GTA582GTT (7520A>T), AGA583AAA (7522G>A), AGT585TCT (7527A>T 7528G>C), GCC587GCA (7535C>A), TTA589CTA (7539T>C), GAG591GAA (7547G>A), CTC593TTA (7551C>T 7553C>A), TCT594GAC (7554T>G 7555C>A 7556T>C), CAG595AAA (7557C>A 7559G>A), AGA596CGA (7560A>C), CAA597AAA (7563C>A), CTA599GAA (7569C>G 7570T>A), TTT600AGA (7572T>A 7573T>G 7574T>A)
Proteins
100510534.1%25883.0%48 (98.0%)38 (77.6%)0/1/0/00
Protein mutations:I1022L (5284A>T 5286C>A), L1031M (5311C>A 5313T>G), A1032E (5315C>A 5316A>G), S1035A (5323T>G), R1036del (5326_5328delAGG), D1037N (5329G>A 5331C>T), L1040V (5338C>G), L1044Y (5350C>T 5351T>A 5352A>C), Y1047F (5360A>T 5361T>C), R1048G (5362C>G 5364A>G)
Codon mutations:TGT1005TGC (5235T>C), AGA1008AGG (5244A>G), GGT1013GGA (5259T>A), GAA1016GAG (5268A>G), AGA1017AGG (5271A>G), AGG1020CGG (5278A>C), ACA1021ACC (5283A>C), ATC1022TTA (5284A>T 5286C>A), AAG1023AAA (5289G>A), ACT1025ACC (5295T>C), ACG1030ACT (5310G>T), CTT1031ATG (5311C>A 5313T>G), GCA1032GAG (5315C>A 5316A>G), TCT1035GCT (5323T>G), AGG1036del (5326_5328delAGG), GAC1037AAT (5329G>A 5331C>T), CTC1040GTC (5338C>G), CTG1041CTT (5343G>T), CTT1042CTC (5346T>C), CTA1044TAC (5350C>T 5351T>A 5352A>C), GCC1045GCT (5355C>T), TAT1047TTC (5360A>T 5361T>C), CGA1048GGG (5362C>G 5364A>G), CGC1050CGT (5370C>T), ACG1052ACC (5376G>C)
5596036.6%25382.7%45 (100%)36 (80.0%)0/0/0/00
Protein mutations:E571Q (7485G>C), T579S (7509A>T), L581V (7515C>G 7517A>G), R583K (7522G>A), S594D (7554T>G 7555C>A 7556T>C), Q595K (7557C>A 7559G>A), Q597K (7563C>A), L599E (7569C>G 7570T>A), F600R (7572T>A 7573T>G 7574T>A)
Codon mutations:AAA560AAG (7454A>G), GAG561GAA (7457G>A), GGA562GGC (7460A>C), GGA563GGG (7463A>G), CTG564CTC (7466G>C), GCT566GCA (7472T>A), CTA568CTC (7478A>C), GAA571CAA (7485G>C), TGT573TGC (7493T>C), TTC574TTT (7496C>T), TAT575TAC (7499T>C), GCT576GCA (7502T>A), ACA579TCA (7509A>T), GGC580GGA (7514C>A), CTA581GTG (7515C>G 7517A>G), GTA582GTT (7520A>T), AGA583AAA (7522G>A), AGT585TCT (7527A>T 7528G>C), GCC587GCA (7535C>A), TTA589CTA (7539T>C), GAG591GAA (7547G>A), CTC593TTA (7551C>T 7553C>A), TCT594GAC (7554T>G 7555C>A 7556T>C), CAG595AAA (7557C>A 7559G>A), AGA596CGA (7560A>C), CAA597AAA (7563C>A), CTA599GAA (7569C>G 7570T>A), TTT600AGA (7572T>A 7573T>G 7574T>A)
*: Inserts / Deletes / Misaligned / Frameshifts

Analysis details

This analysis was performed with panviral2.43