NGS Details (SRR3458562_Metagenome_example): Hubei picorna-like virus 22

Assembly

136 (1 contig(s))
1.0
1
0.06 rpm (after QC)
0
de novo + reference guided assembly
Bcf Tools

Coverage Map

Variant Tables

Assignment

Hubei picorna-like virus 22 (Taxonomy ID: 1923102)
77.9412
84.4444
0
2

Alignment

152.0 (NT) + 318.0 (AA) = 470.0
76.6721
Global, seeded, nucleotide + amino acids (AGA)

Alignment Detailed Statistics

BeginEndCoverageScoreConcordanceMatchesIdentitiesI/D/M/F*Stop Codons
NT476348981.6%15255.9%136 (100%)106 (77.9%)0/0
Mutations:4764A>T, 4770T>C, 4771C>G, 4772A>C, 4773G>C, 4775G>A, 4776C>A, 4782A>G, 4783G>A, 4794T>C, 4803C>G, 4806C>G, 4809T>C, 4812G>A, 4818T>C, 4819T>A, 4821G>T, 4828A>C, 4829A>G, 4839C>T, 4842T>C, 4854C>G, 4860A>C, 4875C>A, 4878G>A, 4881A>C, 4882A>G, 4884T>G, 4890C>T, 4893T>A
CDS
144314872.7%31889.6%45 (100%)38 (84.4%)0/0/0/00
Protein mutations:Q1445A (4771C>G 4772A>C 4773G>C), R1446Q (4775G>A 4776C>A), E1449K (4783G>A), L1461I (4819T>A 4821G>T), K1464R (4828A>C 4829A>G), I1482V (4882A>G 4884T>G)
Codon mutations:GCA1442.CT (4764A>T), AAT1444AAC (4770T>C), CAG1445GCC (4771C>G 4772A>C 4773G>C), CGC1446CAA (4775G>A 4776C>A), CGA1448CGG (4782A>G), GAA1449AAA (4783G>A), TTT1452TTC (4794T>C), GTC1455GTG (4803C>G), GTC1456GTG (4806C>G), CAT1457CAC (4809T>C), TCG1458TCA (4812G>A), CAT1460CAC (4818T>C), TTG1461ATT (4819T>A 4821G>T), AAA1464CGA (4828A>C 4829A>G), AAC1467AAT (4839C>T), TCT1468TCC (4842T>C), TCC1472TCG (4854C>G), CCA1474CCC (4860A>C), GGC1479GGA (4875C>A), ACG1480ACA (4878G>A), GCA1481GCC (4881A>C), ATT1482GTG (4882A>G 4884T>G), AAC1484AAT (4890C>T), TCT1485TCA (4893T>A)
Proteins
144314872.7%31889.6%45 (100%)38 (84.4%)0/0/0/00
Protein mutations:Q1445A (4771C>G 4772A>C 4773G>C), R1446Q (4775G>A 4776C>A), E1449K (4783G>A), L1461I (4819T>A 4821G>T), K1464R (4828A>C 4829A>G), I1482V (4882A>G 4884T>G)
Codon mutations:GCA1442.CT (4764A>T), AAT1444AAC (4770T>C), CAG1445GCC (4771C>G 4772A>C 4773G>C), CGC1446CAA (4775G>A 4776C>A), CGA1448CGG (4782A>G), GAA1449AAA (4783G>A), TTT1452TTC (4794T>C), GTC1455GTG (4803C>G), GTC1456GTG (4806C>G), CAT1457CAC (4809T>C), TCG1458TCA (4812G>A), CAT1460CAC (4818T>C), TTG1461ATT (4819T>A 4821G>T), AAA1464CGA (4828A>C 4829A>G), AAC1467AAT (4839C>T), TCT1468TCC (4842T>C), TCC1472TCG (4854C>G), CCA1474CCC (4860A>C), GGC1479GGA (4875C>A), ACG1480ACA (4878G>A), GCA1481GCC (4881A>C), ATT1482GTG (4882A>G 4884T>G), AAC1484AAT (4890C>T), TCT1485TCA (4893T>A)
*: Inserts / Deletes / Misaligned / Frameshifts

Analysis details

This analysis was performed with panviral2.43