NGS Details (SRR3458562_Metagenome_example): Canine parvovirus

Assembly

5314 (1 contig(s))
24812.6
1232600
79532.83 rpm (after QC)
0
read mapping against reference + variant calling
Bcf Tools

Coverage Map

Variant Tables

Assignment

Canine parvovirus (Taxonomy ID: 10788)
99.304
99.1269
4
3

Alignment

10441.0 (NT) + 10703.0 (AA) = 21144.0
98.8499
Global, seeded, nucleotide + amino acids (AGA)

Alignment Detailed Statistics

BeginEndCoverageScoreConcordanceMatchesIdentitiesI/D/M/F*Stop Codons
NT1531799.8%1044198.5%5312 (99.9%)5279 (99.3%)4/2
Mutations:48_49insGC, 49C>G, 67A>G, 258C>T, 267C>T, 436A>G, 517T>C, 721A>G, 1534T>C, 2078G>A, 2197G>C, 2344G>A, 2381T>C, 2920C>T, 3024G>A, 3064C>A, 3087T>C, 3093C>T, 3469A>G, 3479A>G, 3752A>G, 3884T>G, 4060G>A, 4270T>C, 4408C>A, 4476G>A, 4488G>C, 4593delA, 4603T>C, 4616delG, 4656_4657insT, 4679G>A, 4701_4702insG, 4709G>T, 4822T>A, 4913C>T, 5249G>A, 5261G>C
CDS
1669100%482699.8%669 (100%)668 (99.9%)0/0/0/01
Protein mutations:D603N (2078G>A)
Codon mutations:GAA55GAG (436A>G), GAT82GAC (517T>C), CAA150CAG (721A>G), GGT421GGC (1534T>C), GAC603AAC (2078G>A), ACG642ACC (2197G>C)
1752100%540898.8%752 (100%)742 (98.7%)0/0/0/02
Protein mutations:F33L (2381T>C), R247K (3024G>A), N260K (3064C>A), I268T (3087T>C), A270V (3093C>T), I399V (3479A>G), N490D (3752A>G), Y534D (3884T>G), S731N (4476G>A), G735A (4488G>C)
Codon mutations:GAG20GAA (2344G>A), TTT33CTT (2381T>C), TTC212TTT (2920C>T), AGA247AAA (3024G>A), AAC260AAA (3064C>A), ATT268ACT (3087T>C), GCA270GTA (3093C>T), ACA395ACG (3469A>G), ATA399GTA (3479A>G), AAC490GAC (3752A>G), TAT534GAT (3884T>G), ACG592ACA (4060G>A), CCT662CCC (4270T>C), GCC708GCA (4408C>A), AGT731AAT (4476G>A), GGT735GCT (4488G>C)
168100%46997.5%68 (100%)66 (97.1%)0/0/0/01
Protein mutations:S47N (5249G>A), G51A (5261G>C)
Codon mutations:AGT47AAT (5249G>A), GGT51GCT (5261G>C)
Proteins
1669100%482699.8%669 (100%)668 (99.9%)0/0/0/01
Protein mutations:D603N (2078G>A)
Codon mutations:GAA55GAG (436A>G), GAT82GAC (517T>C), CAA150CAG (721A>G), GGT421GGC (1534T>C), GAC603AAC (2078G>A), ACG642ACC (2197G>C)
1752100%540898.8%752 (100%)742 (98.7%)0/0/0/02
Protein mutations:F33L (2381T>C), R247K (3024G>A), N260K (3064C>A), I268T (3087T>C), A270V (3093C>T), I399V (3479A>G), N490D (3752A>G), Y534D (3884T>G), S731N (4476G>A), G735A (4488G>C)
Codon mutations:GAG20GAA (2344G>A), TTT33CTT (2381T>C), TTC212TTT (2920C>T), AGA247AAA (3024G>A), AAC260AAA (3064C>A), ATT268ACT (3087T>C), GCA270GTA (3093C>T), ACA395ACG (3469A>G), ATA399GTA (3479A>G), AAC490GAC (3752A>G), TAT534GAT (3884T>G), ACG592ACA (4060G>A), CCT662CCC (4270T>C), GCC708GCA (4408C>A), AGT731AAT (4476G>A), GGT735GCT (4488G>C)
126100%176100%26 (100%)26 (100%)0/0/0/00
Protein mutations:none
Codon mutations:GAG20GAA (2344G>A)
1585100%424798.7%585 (100%)576 (98.5%)0/0/0/00
Protein mutations:R81K (3024G>A), N94K (3064C>A), I102T (3087T>C), A104V (3093C>T), I233V (3479A>G), N324D (3752A>G), Y368D (3884T>G), S565N (4476G>A), G569A (4488G>C)
Codon mutations:TTC46TTT (2920C>T), AGA81AAA (3024G>A), AAC94AAA (3064C>A), ATT102ACT (3087T>C), GCA104GTA (3093C>T), ACA229ACG (3469A>G), ATA233GTA (3479A>G), AAC324GAC (3752A>G), TAT368GAT (3884T>G), ACG426ACA (4060G>A), CCT496CCC (4270T>C), GCC542GCA (4408C>A), AGT565AAT (4476G>A), GGT569GCT (4488G>C)
168100%46997.5%68 (100%)66 (97.1%)0/0/0/01
Protein mutations:S47N (5249G>A), G51A (5261G>C)
Codon mutations:AGT47AAT (5249G>A), GGT51GCT (5261G>C)
*: Inserts / Deletes / Misaligned / Frameshifts

Analysis details

This analysis was performed with panviral2.43